Full TGIF Record # 159799
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Web URL(s):http://archive.lib.msu.edu/tic/ressum/2009/9.pdf
    Last checked: 01/23/2017
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Publication Type:
Author(s):Lakshman, Dilip K.
Author Affiliation:USDA-ARS
Title:Accurate identification and gene expression in relation to virulence of Rhizoctonia isolates infecting turfgrasses
Section:Integrated turfgrass management
Other records with the "Integrated turfgrass management" Section
Source:2009 Turfgrass and Environmental Research Summary. 2009, p. 9.
Publishing Information:Far Hills, NJ: United States Golf Association
# of Pages:1
Keywords:TIC Keywords: Disease identification; Gene expression; Rhizoctonia blight; Rhizoctonia cerealis; Rhizoctonia solani; Rhizoctonia zeae; Waitea circinata var. zeae
Abstract/Contents:Presents a study conducted to determine the "molecular identification of Rhizoctonia solania isolates pathogenic to turfgrasses using Universally Primed-Polymerase Chain reaction (UP-PCR) and nucleic acid hybridization analysis." Describes the experiment and the methods used, explaining that "we collected a total of 448 Rhizoctonia samples from 5 locations in northern Virginia and Maryland during the summer of 2008 and 2009."
See Also:Other Reports from this USGA research project: 2007-10-351
Note:Pictures, b/w
USGA Summary Points:We have collected a total of 448 Rhizoctonia isolates from golf courses and lawns of Virginia and Maryland. Analyzing the rDNA-ITS sequence variations of 54 randomly sampled isolates, we found that majority (i.e., 61%) of the isolates belong to R. solani, while 22% belong to R. zeae/W. cercinata var circinata, and 17% belong to R. cerealis/Rhizoctonia-like fungi. Of the identified R. solani isolates in this study, majority belong to AG2-2IIIB and AG 1-1B. Genome fingerprinting of Rhizoctonia isolates using UP-PCR and AFLP techniques are in progress. Analyzing the two virulence-differentiated EST libraries, we have identified 532 and 495 unigenes of R. solani AG-4. Among several indentified genes are thsoe with possible roles as elicitors of disease, in cell wall degradation, avoidance of host and drug resistance and mating.
ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Lakshman, D. K. 2009. Accurate identification and gene expression in relation to virulence of Rhizoctonia isolates infecting turfgrasses. Turfgrass Environ. Res. Summ. p. 9.
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    Last checked: 01/23/2017
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