Full TGIF Record # 159906
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Web URL(s):http://archive.lib.msu.edu/tic/ressum/2009/61.pdf
    Last checked: 01/23/2017
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Publication Type:
i
Report
Author(s):Richmond, Douglas; Schemerhorn, Brandon; Abdelwahab, Mohamed
Author Affiliation:Purdue University
Title:Unraveling billbug seasonal ecology to improve management: Developing a DNA-based larval identification tool
Section:Grant-in-Aid Research Program
Other records with the "Grant-in-Aid Research Program" Section
Source:2009 Turfgrass and Environmental Research Summary. 2009, p. 61.
Publishing Information:Far Hills, NJ: United States Golf Association
# of Pages:1
Keywords:TIC Keywords: DNA; Ecology; Identification; Larva; Sphenophorus
Abstract/Contents:Presents a study conducted to "identify key regions in billbug rDNA sequences that can be used to identify and differentiate major billbug pest species." Describes the methods and materials used in the experiment, explaining that "several hundred billbug adult and larval specimens were collected from various locations across the U.S. [United States]. Specimens include all known billbug pest species."
Language:English
References:0
See Also:Other Reports from this USGA research project: 2009-06-398
Note:Pictures, color
USGA Summary Points:A DNA-based billbug larval identification tool could provide researchers with the means to gain a more complete understanding of the seasonal biology of this emerging pest complex on a region-by-region basis, leading to improved management programs. The ITS2 region of the rDNA multigene family will allow differentiation between S. parvulus (338 base pairs) and S. minimus (463 base pairs) based on the size (number of base pairs) of the region alone. ITS2 will allow us to dependably differentiate these two species from S. cicatristriatus, S. venatus, and S. inaequalis, all of which share an ITS2 region of identical size (348 base pairs) and sequence. Because ITS2 does not provide the differences in size or sequence necessary to dependably differentiate all billbug species of concern, future efforts will focus on other regions within the ribosomal RNA multigene family (ITS1, CO1, and IGS).
ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Richmond, D., B. Schemerhorn, and M. Abdelwahab. 2009. Unraveling billbug seasonal ecology to improve management: Developing a DNA-based larval identification tool. Turfgrass Environ. Res. Summ. p. 61.
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http://archive.lib.msu.edu/tic/ressum/2009/61.pdf
    Last checked: 01/23/2017
    Requires: PDF Reader
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