Full TGIF Record # 173870
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Web URL(s):http://archive.lib.msu.edu/tic/ressum/2010/39.pdf
    Last checked: 01/23/2017
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Publication Type:
i
Report
Author(s):Richmond, Douglas; Schemerhorn, Brandon; Abdelwahab, Mohamed
Author Affiliation:Purdue University
Title:Unraveling billbug seasonal ecology to improve management: Developing a DNA-based larval identification tool
Section:Grant-in-aid research program
Other records with the "Grant-in-aid research program" Section
Source:2010 Turfgrass and Environmental Research Summary. 2010, p. 39.
Publishing Information:Far Hills, NJ: United States Golf Association
# of Pages:1
Keywords:TIC Keywords: Analytical methods; DNA; Genetic markers; Genetics; Insect identification; Larva; Polymerase chain reaction; Sphenophorus
Abstract/Contents:Presents a study initiated during 2009 to "(1) identify key regions in billbug rDNA [recombinant deoxyribonucleic acid] sequences that can be used to identify and differentiate major billbug pest species" and "(2) create species-specific DNA primers for these same rDNA regions that will allow for rapid and dependable identification of field-collected billbug larvae." Details methods and materials used during the experiment, noting that "adult [billbug] specimens were identified to species using classic morphological characteristics. After the identity of adult specimens was confirmed, several regions...of ribosomal DNA were extracted, amplified, and sequenced." Reports that CO1, the second rDNA region examined, did not prove at all useful in differentiating billbug species as the size and sequence of this region was identical for all species." Concludes that "when [the study is] complete, DNA primers can be developed for use in a multiplex PCR [polymerase chain reaction] system that will allow accurate identification of field collected billbug larvae, facilitate studies of billbug seasonal ecology, and improve billbug management."
Language:English
References:0
See Also:Other Reports from this USGA research project: 2009-06-398
Note:Published as a part of USGA Turfgrass and Environmental Research Online, December 1 2010, Vol. 9, No. 23
Pictures, color
USGA Summary Points:A DNA-based billbug larval identification tool could provide researchers with the means to gain a more complete understanding of the seasonal biology of the billbug pest complex and provide a science-based foundation for improving management programs. The ITS2 region of the rDNA will allow differentiation between S. Parvulus and S. Minimus as well as differentiation of these two species from all other species examined. Although CO1 was not useful for differentiating any of the species examined, ITS1 will likely provide further resolution between species as the ITS1 sequence for three of the five species examined to date varies significantly in size. When complete, DNA primers can be developed for use in a multiplex PCR system that will allow accurate identification of field collected billbug larvae, facilitate studies of billbug seasonal ecology, and improve billbug management.
ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Richmond, D., B. Schemerhorn, and M. Abdelwahab. 2010. Unraveling billbug seasonal ecology to improve management: Developing a DNA-based larval identification tool. Turfgrass Environ. Res. Summ. p. 39.
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http://archive.lib.msu.edu/tic/ressum/2010/39.pdf
    Last checked: 01/23/2017
    Requires: PDF Reader
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