| |
| DOI: | 10.1371/journal.pone.0171183 |
|---|
| Web URL(s): | http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0171183
Last checked: 04/04/2017
http://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0171183&type=printable
Last checked: 04/04/2017
Requires: PDF Reader |
|---|
| Publication Type:
| Refereed |
|---|
| Author(s): | Jespersen, David;
Belanger, Faith C.;
Huang, Bingru |
|---|
| Author Affiliation: | Jespersen: Department of Plant Biology and Pathology, Rutgers University, New Brunswick, New Jersey and Department of Crop and Soil Sciences, University of Georgia, Griffin, Georgia; Belanger and Huang: Department of Plant Biology and Pathology, Rutgers University, New Brunswick, New Jersey |
|---|
| Title: | Candidate genes and molecular markers associated with heat tolerance in colonial bentgrass |
|---|
| Source: | PloS ONE. Vol. 12, No. 2, February 10 2017, p. 1-17. |
|---|
| Publishing Information: | San Francisco, CA: Public Library of Science |
|---|
| # of Pages: | 17 |
|---|
| Keywords: | TIC Keywords: Agrostis tenuis; Cool season turfgrasses; Gene expression; Heat resistance; Molecular markers; Phenotypes; Stress tolerance
|
|---|
| Abstract/Contents: | "Elevated temperature is a major abiotic stress limiting the growth of cool-season grasses during the summer months. The objectives of this study were to determine the genetic variation in the expression patterns of selected genes involved in several major metabolic pathways regulating heat tolerance for two genotypes contrasting in heat tolerance to confirm their status as potential candidate genes, and to identify PCR-based markers associated with candidate genes related to heat tolerance in a colonial (Agrostis capillaris L.) x creeping bentgrass (Agrostis stolonifera L.) hybrid backcross population. Plants were subjected to heat stress in controlled-environmental growth chambers for phenotypic evaluation and determination of genetic variation in candidate gene expression. Molecular markers were developed for genes involved in protein degradation (cysteine protease), antioxidant defense (catalase and glutathione-S-transferase), energy metabolism (glyceraldehyde-3-phosphate dehydrogenase), cell expansion (expansin), and stress protection (heat shock proteins HSP26, HSP70, and HSP101). Kruskal-Wallis analysis, a commonly used non-parametric test used to compare population individuals with or without the gene marker, found the physiological traits of chlorophyll content, electrolyte leakage, normalized difference vegetative index, and turf quality were associated with all candidate gene markers with the exception of HSP101. Differential gene expression was frequently found for the tested candidate genes. The development of candidate gene markers for important heat tolerance genes may allow for the development of new cultivars with increased abiotic stress tolerance using marker-assisted selection." |
|---|
| Language: | English |
|---|
| References: | 56 |
|---|
| Note: | Figures
Tables
Graphs |
|---|
| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Jespersen, D., F. C. Belanger, and B. Huang. 2017. Candidate genes and molecular markers associated with heat tolerance in colonial bentgrass. PloS ONE. 12(2):p. 1-17. |
| Fastlink to access this record outside TGIF: https://tic.lib.msu.edu/tgif/flink?recno=282221 |
| If there are problems with this record, send us feedback about record 282221. |
| Choices for finding the above item: |
|---|
| DOI: 10.1371/journal.pone.0171183 |
| Web URL(s):
http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0171183
Last checked: 04/04/2017
http://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0171183&type=printable
Last checked: 04/04/2017
Requires: PDF Reader |
| 
MSU catalog number: b5304007 |
| Find from within TIC:
Digitally in TIC by record number. |
| Request through your local library's inter-library loan service (bring or send a copy of this TGIF record) |
