| |
| DOI: | 10.1186/s12866-016-0623-9 |
|---|
| Web URL(s): | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4715354/pdf/12866_2016_Article_623.pdf
Last checked: 4/4/2017
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4715354/?report=classic
Last checked: 4/4/2017 |
|---|
| Publication Type:
| Refereed |
|---|
| Author(s): | Shehata, Hanan R.;
Lyons, Eric M.;
Jordan, Katerina S.;
Raizada, Manish N. |
|---|
| Author Affiliation: | Jordan and Raizada: Department of Plant Agriculture, University of Guelph, Guelph, ON, Canada; Lyons: Department of Microbiology, School of Pharmacy, Mansoura University, Mansoura, Egypt; Shehata: Department of Plant Agriculture, University of Guelph, Guelph, ON, Canada and Department of Microbiology, School of Pharmacy, Mansoura University, Mansoura, Egypt |
|---|
| Title: | Relevance of in vitro agar based screens to characterize the anti-fungal activities of bacterial endophyte communities |
|---|
| Source: | BMC Microbiology. Vol. 16, 2016, p. 1-7. |
|---|
| Publishing Information: | London, United Kingdom: BioMed Central |
|---|
| # of Pages: | 7 |
|---|
| Keywords: | TIC Keywords: Agrostis stolonifera; Biological control; Culture techniques; Endophytes; In vitro; Microbial activity; Pathogenic fungi; Rhizoctonia solani; Sclerotinia homoeocarpa
|
|---|
| Abstract/Contents: | "Background: Endophytes are microbes that inhabit internal plant tissues without causing disease. Plant microbial communities consist of large numbers of endophyte species. Understanding the functions of these endophytes is a major challenge. An important function of some endophytes is to suppress fungal pathogens. Typically, plant associated microbes are screened for anti-fungal activities in vitro using the high-throughput dual culture screen, but it is not clear whether this method correlates with the activities of these microbes in planta. Furthermore, it is not clear whether in vitro screening captures all of the microbes that show this activity inside plants. The objective of this study was to evaluate the relevance of the in vitro dual culture method for screening endophytes with anti-fungal activity. Results: In parallel, 190 bacterial endophytes from the corn grass family (Zea) were screened for suppression of two fungal pathogens (Sclerotinia homoeocarpa and Rhizoctonia solani) using the in vitro dual culture method, and in planta using the model plant, creeping bentgrass. All endophytes that showed anti-fungal activity in planta against Sclerotinia homoeocarpa and Rhizoctonia solani (3 or 4 strains, respectively, out of 190), were captured in vitro. The in vitro and in planta screening results strongly correlated (r = 0.81 and r = 0.94 for the two pathogens, respectively). Conclusions: Evidence was gained here that the in vitro dual culture method is a relevant method for high throughput screening of plant endophyte communities for anti-fungal activity. In our study, the method captured all of the microbes that suppressed the corresponding pathogens in planta." |
|---|
| Language: | English |
|---|
| References: | 29 |
|---|
| Note: | Pictures, color
Graphs |
|---|
| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Shehata, H. R., E. M. Lyons, K. S. Jordan, and M. N. Raizada. 2016. Relevance of in vitro agar based screens to characterize the anti-fungal activities of bacterial endophyte communities. BMC Microbiol. 16:p. 1-7. |
| Fastlink to access this record outside TGIF: https://tic.lib.msu.edu/tgif/flink?recno=282426 |
| If there are problems with this record, send us feedback about record 282426. |
| Choices for finding the above item: |
|---|
| DOI: 10.1186/s12866-016-0623-9 |
| Web URL(s):
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4715354/pdf/12866_2016_Article_623.pdf
Last checked: 4/4/2017
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4715354/?report=classic
Last checked: 4/4/2017 |
| 
MSU catalog number: b4881806 |
| Find from within TIC:
Digitally in TIC by record number. |
| Request through your local library's inter-library loan service (bring or send a copy of this TGIF record) |
