Full TGIF Record # 305011
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Web URL(s):http://archive.lib.msu.edu/tic/ressum/2018/2018.pdf#page=271
    Last checked: 05/02/2019
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Author(s):Koch, Paul; Clarke, Bruce; Murphy, Jim; Jung, Geunhwa; Zhang, Ning
Author Affiliation:Koch: Department of Plant Pathology, University of Wisconsin-Madison; Clarke, Murphy, and Zhang: Department of Plant Biology and Pathology, Rutgers University; Jung: Stockbridge School of Agriculture, University of Massachusetts-Amherst
Title:Sclerotinia homoeocarpa epidemiology and resistance development as measured through improved molecular detection techniques
Section:Integrated turfgrass management
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Source:Turfgrass and Environmental Research Program: 2018 Research Summaries. 2018, p. 263-273.
Publishing Information:[New York, New York]: The United States Golf Association Green Section
# of Pages:11
Keywords:TIC Keywords: Disease resistance; Epidemiology; Fungicide application; Genetic analysis; Sclerotinia homoeocarpa
See Also:Other Reports from this USGA research project: 2018-20-670
Note:Pictures, b/w
USGA Summary Points:Dollar spot sampling sites were established in New Jersey, Wisconsin, and Massachusetts and sampled repeatedly throughout the 2018 growing season. Two assays are in development for quantifying the dollar spot fungus in field samples. The first is a digital droplet PCR (ddPCR) assay being developed at Wisconsin that is very new to Plant Pathology and represents a potential significant advancement for the detection of fungal DNA in field samples. The second is a more common quantitative PCR (qPCR) assay being developed at Rutgers and Massachusetts that will provide an assay that nearly any research laboratory would be able to perform without the specialized equipment required of ddPCR. The ddPCR protocol developed at Wisconsin is highly sensitive and repeatable and appears to lessen some of the variability and noise inherent to so many field DNA detection techniques. Wisconsin is currently using the ddPCR protocol to assay the field samples collected throughout 2018. The first version of the qPCR protocol developed at Rutgers was not specific enough to reliably differentiate non-treated controls from field samples, and a new and more specific assay is currently being developed. Assessment of the 2018 samples collected from New Jersey will be conducted once the new qPCR assay has been developed. Massachusetts also used a qPCR assay and was able to consistently detect the dollar spot fungus in the thatch of samples collected in the field. The fungal population did not appear to change in size throughout the 2018 season. Field studies using the respective assays described above that investigate the impact of various chemical and cultural practices on dollar spot pathogen development will be planned early in 2019 for implementation during the 2019 growing season. A molecular diagnostic system for dollar spot was developed at Massachusetts to detect mutations that confer resistance to SDHI fungicides. This system will be tested on more field isolates from Massachusetts, New Jersey, and Wisconsin in 2019.
ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Koch, P., B. Clarke, J. Murphy, and G. Jung. 2018. Sclerotinia homoeocarpa epidemiology and resistance development as measured through improved molecular detection techniques. USGA Turfgrass Environ. Res. Summ. p. 263-273.
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    Last checked: 05/02/2019
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