Full TGIF Record # 72140
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Web URL(s):http://archive.lib.msu.edu/tic/ressuml/210.pdf
    Last checked: 01/20/2017
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Publication Type:
i
Report
Author(s):Hughes, Harrison G.
Author Affiliation:Colorado State University
Title:Long-term preservation of clonally propagated turfgrass species
Section:Turfgrass germplasm enhancement
Other records with the "Turfgrass germplasm enhancement" Section
Source:2000 Turfgrass and Environmental Research Summary [USGA]. 2000, p. 38.
Publishing Information:Far Hills, NJ: United States Golf Association
# of Pages:1
Full Report URL:http://turf.lib.msu.edu/rprl/616.pdf
    Last checked: 9/2001
    Requires: Adobe Acrobat
    Notes: This is the entire full report!
Keywords:TIC Keywords: Germplasm; Polycross progeny; Micropropagation; Cynodon dactylon; Zoysia; Distichlis spicata; Bouteloua dactyloides; Root tips; Germination media; Tissue culture; Cryogenic endodormancy; Cryogenic paradormancy; Freezing; Timing; Golf courses
Abstract/Contents:Presents a study with the following objectives: "1. Develop suitable micropropagation procedures for selected genotypes of bermudagrass, zoysiagrass, saltgrass and buffalograss. 2. Develop suitable shoot tip culture media (SCTM) for the four species. 3. Examine cryopreservation of the four species using vitrificiation methodologies." States that "three cryopreservation methods are being tested: two-step cooling, vitrification, and encapsulation/dehydration." Reports that although clean cultures have been established with both saltgrass and bermudagrass, the proliferation rates are minimal." Also, "buffalograss has been cryopreserved with the vitrification system." This three-year study began in 1998.
Language:English
References:0
See Also:See also related manuscript, Long-Term Preservation of Clonally Propagated Turfgrass Species, 2000, R=215147. R=215147
See Also:Other Reports from this USGA research project: 1998-20-128
Note:Pictures, color
USGA Summary Points:Three cryopreservation methods are being tested: two-step cooling, vitrification, and encapsulation/dehydration. Cooling rate was important - more rapidly cooled shoot tips showed higher levels of survival. Attention to quality of shoot tips is important - need axillary meristems. For buffalograss, vitrification had worked the best. Prograss is being made to cryopreserve bermudagrass and saltgrass. Micropropagation systems for buffalograss and zoysiagrass are needed that will allow sufficient proliferation rates for cryopreservation studies.
ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Hughes, H. G. 2000. Long-term preservation of clonally propagated turfgrass species. Turfgrass Environ Res. Summ. p. 38.
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Web URL(s):
http://turf.lib.msu.edu/rprl/616.pdf
    Last checked: 9/2001
    Requires: Adobe Acrobat
    Notes: This is the entire full report!
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MSU catalog number: SB 433 .A1 A6
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