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Web URL(s): | http://archive.lib.msu.edu/tic/ressuml/212.pdf Last checked: 01/20/2017 Requires: PDF Reader |
Publication Type:
| Report |
Author(s): | Qu, Rongda |
Author Affiliation: | North Carolina State University |
Title: | Hybrid bermudagrass improvement by genetic transformation |
Section: | Turfgrass germplasm enhancement Other records with the "Turfgrass germplasm enhancement" Section
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Source: | 2000 Turfgrass and Environmental Research Summary [USGA]. 2000, p. 40. |
Publishing Information: | Far Hills, NJ: United States Golf Association |
# of Pages: | 1 |
Full Report URL: | http://turf.lib.msu.edu/rprl/620.pdf Last checked: 9/2001 Requires: Adobe Acrobat Notes: This is the entire full report! |
Keywords: | TIC Keywords: Golf courses; Hybrid bermudagrasses; Cultivar improvement; Genetic transformation; Tissue culture; Regeneration; Embryogenesis; Calli; Biolistic transformation; Transgenic turfgrasses; Plant recovery; Nematode control; Genetic engineering; Somatic embryogenesis; Gibberellic acid; Agrobacterium tumefaciens
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Abstract/Contents: | Presents a study with the following objectives: "1. Develop and optimize tissue culture conditions in order to obtain embryogenic callus and regeneration of hybrid bermudagrass plantlets. 2. Develop a procedure to transform the embryogenic callus by the biolistic (particle bombardment) method and to recover transgenic plants. 3. Obtain transgenic plants of hybrid bermudagrass that express nematode-resistance genes." States that "so far, we have spent substantial efforts to improve the tissue culture responses of bermudagrass...Addition of gibberellic acid further improved the generation/regeneration of embryos." This five-year study began in 1998. |
Language: | English |
References: | 0 |
See Also: | See also related manuscript, Bermudagrass Improvement by Genetic Transformation, 2000, R=215154. R=215154 |
See Also: | Other Reports from this USGA research project: 1998-18-126 |
Note: | Pictures, color |
USGA Summary Points: | It is difficult to derive embryogenic callus from bermudagrass. Supplementing BAP and ABA in the callus-induction media improved somatic embryogenesis. Giberellic acid further improved the generation/regenteration of embryos. Secondary somatic embryogenesis was observed. Agrobacterium-mediated transformation is being developed. The GUS-reporter gene is showing activity in five cell suspension lines. |
| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete): Qu, R. 2000. Hybrid bermudagrass improvement by genetic transformation. Turfgrass Environ Res. Summ. p. 40. |
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| Web URL(s): http://turf.lib.msu.edu/rprl/620.pdf Last checked: 9/2001 Requires: Adobe Acrobat Notes: This is the entire full report! |
| MSU catalog number: SB 433 .A1 A6 |
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