Full TGIF Record # 72142
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Web URL(s):http://archive.lib.msu.edu/tic/ressuml/212.pdf
    Last checked: 01/20/2017
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Publication Type:
i
Report
Author(s):Qu, Rongda
Author Affiliation:North Carolina State University
Title:Hybrid bermudagrass improvement by genetic transformation
Section:Turfgrass germplasm enhancement
Other records with the "Turfgrass germplasm enhancement" Section
Source:2000 Turfgrass and Environmental Research Summary [USGA]. 2000, p. 40.
Publishing Information:Far Hills, NJ: United States Golf Association
# of Pages:1
Full Report URL:http://turf.lib.msu.edu/rprl/620.pdf
    Last checked: 9/2001
    Requires: Adobe Acrobat
    Notes: This is the entire full report!
Keywords:TIC Keywords: Golf courses; Hybrid bermudagrasses; Cultivar improvement; Genetic transformation; Tissue culture; Regeneration; Embryogenesis; Calli; Biolistic transformation; Transgenic turfgrasses; Plant recovery; Nematode control; Genetic engineering; Somatic embryogenesis; Gibberellic acid; Agrobacterium tumefaciens
Abstract/Contents:Presents a study with the following objectives: "1. Develop and optimize tissue culture conditions in order to obtain embryogenic callus and regeneration of hybrid bermudagrass plantlets. 2. Develop a procedure to transform the embryogenic callus by the biolistic (particle bombardment) method and to recover transgenic plants. 3. Obtain transgenic plants of hybrid bermudagrass that express nematode-resistance genes." States that "so far, we have spent substantial efforts to improve the tissue culture responses of bermudagrass...Addition of gibberellic acid further improved the generation/regeneration of embryos." This five-year study began in 1998.
Language:English
References:0
See Also:See also related manuscript, Bermudagrass Improvement by Genetic Transformation, 2000, R=215154. R=215154
See Also:Other Reports from this USGA research project: 1998-18-126
Note:Pictures, color
USGA Summary Points:It is difficult to derive embryogenic callus from bermudagrass. Supplementing BAP and ABA in the callus-induction media improved somatic embryogenesis. Giberellic acid further improved the generation/regenteration of embryos. Secondary somatic embryogenesis was observed. Agrobacterium-mediated transformation is being developed. The GUS-reporter gene is showing activity in five cell suspension lines.
ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Qu, R. 2000. Hybrid bermudagrass improvement by genetic transformation. Turfgrass Environ Res. Summ. p. 40.
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Web URL(s):
http://turf.lib.msu.edu/rprl/620.pdf
    Last checked: 9/2001
    Requires: Adobe Acrobat
    Notes: This is the entire full report!
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MSU catalog number: SB 433 .A1 A6
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