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| Web URL(s): | http://archive.lib.msu.edu/tic/ressuml/218.pdf
Last checked: 01/20/2017
Requires: PDF Reader |
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| Publication Type:
| Report |
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| Author(s): | Anderson, Michael P. |
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| Author Affiliation: | Oklahoma State University |
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| Title: | Transformation of bermudagrass for improved fungal resistance |
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| Section: | Turfgrass germplasm enhancement
Other records with the "Turfgrass germplasm enhancement" Section
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| Source: | 2000 Turfgrass and Environmental Research Summary [USGA]. 2000, p. 46. |
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| Publishing Information: | Far Hills, NJ: United States Golf Association |
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| # of Pages: | 1 |
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| Full Report URL: | http://turf.lib.msu.edu/rprl/626.pdf
Last checked: 9/2001
Requires: Adobe Acrobat
Notes: This is the entire full report! |
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| Keywords: | TIC Keywords: Disease resistance; Golf courses; Genetic transformation; Cynodon dactylon; Fungus control; Spring dead spot; Genetic engineering; Chitinase; Genetic resistance; Biolistic transformation; Genotypes; Gene expression; Ophiosphaerella herpotricha; Ophiosphaerella korrae
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| Abstract/Contents: | Presents a study with the following objectives: "1. Isolate, identify, and characterize chitinases and glucanases and their genes that have high activity against Spring Dead Spot (SDS). 2. Develop an efficient protocol to transform (genetically engineer) bermudagrass. 3. Transform and characterize bermudagrass with the antifungal chitinase and/or glucanase genes directed against the SDS casual organism." States that "evaluation of all 671 putative transformants indicated that none of these plants were transgenic. New transformation experiments utilizing higher concentrations of bialaphos have been initiated." Reports that "isolation of the factor using preparative SDS-PAGE and reverse-phase chromatography was successful." This five-year study began in 1998. |
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| Language: | English |
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| References: | 0 |
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| See Also: | See also related manuscript, Transformation of Bermudagrass for Improved Fungal Tolerance, 2000, R=215183. R=215183 |
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| See Also: | Other Reports from this USGA research project: 1998-11-119 |
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| Note: | Pictures, color |
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| USGA Summary Points: | Transformation techniques for bermudagrass using biolistic gun are being developed. A turf-type genotype, PRC-58, was identified with tissue culture and plant regeneration capabilities. A sensitive assay was developed to determine the relative infection state of bermudagrass crown tissue. Genomic analysis was initiated during the past year to study gene expression profiles between tolerant (OKS 91-11) and susceptible (Jackpot) varieties when infected with O. herpotricha. A microorganism with potent activity against O. herpotricha was isolated and the anti-fungal factor it produces was stable in vitro for six months. |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Anderson, M. P. 2000. Transformation of bermudagrass for improved fungal resistance. Turfgrass Environ Res. Summ. p. 46. |
| Fastlink to access this record outside TGIF: https://tic.lib.msu.edu/tgif/flink?recno=72148 |
| If there are problems with this record, send us feedback about record 72148. |
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| Web URL(s):
http://turf.lib.msu.edu/rprl/626.pdf
Last checked: 9/2001
Requires: Adobe Acrobat
Notes: This is the entire full report! |
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MSU catalog number: SB 433 .A1 A6 |
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