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| Publication Type:
| Report |
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| Content Type: | Abstract or Summary only |
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| Author(s): | Oprisko, M. J.;
Beard, J. B.;
Green, R. L. |
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| Author Affiliation: | Texas A&M University |
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| Title: | An assessment of vital and mortal stains for research of root hair viability of 12 warm-season perennial grasses. |
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| Source: | Agronomy Abstracts. Vol. 80, November/December 1988, p. 154. |
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| Publishing Information: | Madison, WI: American Society of Agronomy |
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| # of Pages: | 1 |
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| Keywords: | TIC Keywords: Perennial grasses; Staining; Species; Root hairs; Viability
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| Abstract/Contents: | "This research documents the search and discovery of a simple-to-use, reliable and easily detectable vital stain for root hairs of 12 warm-season perennial turfgrasses. Of the 5 vital stains used in this study, methylene blue, Evan's blue, congo red, neutral red and phenosafranin, only Evan's blue consistently gave the same color contrast for live and dead root hairs on the 12 genotypes (6 species) characterized in this survey. In all cases a dead root hair control (boiled) was compared against fresh roots. All the genotypes were established from single sprigs and grown under greenhouse conditions in plastic containers containing sand. While color contrast was confirmed as the indication of live and dead root hairs by the collapse of live root hairs in 20 M sucrose, intensity of color was not. Several of the stains showed inconsistency in the color and intensity of stain uptake by live and dead root hairs. Due to the inconsistency in stain uptake and intensity by root hairs, great emphasis was placed on obtaining an adequate sample size with statistical confirmation. The relative reliability of these stains in descending order was: Evan's blue, methylene blue, phenosafranin, congo red and neutral red with root hairs of 12 C-4 perennial grasses." |
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| Language: | English |
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| References: | 0 |
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| See Also: | See also related manuscript, Fifth Year Progress Report Concerning Physiological Investigations in Developing Water Conserving Minimal Maintenance Turfgrasses and Cultural Systems: Volume V, 1988, R=217144. R=217144 |
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| Note: | This item is an abstract only! |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Oprisko, M. J., J. B. Beard, and R. L. Green. 1988. An assessment of vital and mortal stains for research of root hair viability of 12 warm-season perennial grasses.. Annu. Meet. Abstr. 80:p. 154. |
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MSU catalog number: S 1 .A58 |
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