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| Web URL(s): | http://archive.lib.msu.edu/tic/rpr/2000/72143,%20OK%20State, Taliaferro, Cold Hardiness.PDF
Last checked: 02/11/2013
Requires: PDF Reader |
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| Publication Type:
| Report |
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| Material Type: | Manuscript |
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| Monographic Author(s): | Taliaferro, C. M.; Anderson, Jeffrey A.; Anderson, Michael P.; Tudor, Veronica A.; Martin, Dennis L.; Bell, Gregory E. |
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| Author Affiliation: | Taliaferro: PI, Plant Breeding and Genetics; Anderson, J: Co-PI, Plant Stress Physiology; Anderson, M: Co-PI, Molecular Genetics; Tudor: Research Assistant; and Martin and Bell: Co-PI, Turfgrass Science |
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| Monograph Title: | Bermudagrass Cold Hardiness: Characterization of Plants for Freeze Tolerance and Characterization of Low Temperature-Induced Genes: Annual Report, [2000]. |
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| Publishing Information: | [Far Hills, New Jersey]: United States Golf Association; [Stillwater, Oklahoma]: Oklahoma Agricultural Experiment Station, Oklahoma State University |
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| # of Pages: | 16 |
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| Collation: | [1], 11, [4] |
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| Abstract/Contents: | "Injury to bermudagrass turf caused by freezing temperatures during winter is a persistent problem over much of its geographic area of use in the USA. This research seeks to reduce risk of freeze injury to bermudagrass grown in temperate regions. The research focuses on accurately assessing the freeze tolerance of bermudagrass cultivars, isolating genes responsible for enhanced freeze tolerance, and enhancing knowledge of the fundamental mechanisms associated with cold hardiness. Specific objectives are to: 1) quantify cold-hardiness of advanced breeding lines, recently released varieties, and established standard varieties and 2) isolate and characterize cold regulated (Cor) genes responsible for conferring freeze tolerance. The low temperature tolerance (LT50) of 11 turf bermudagrasses was evaluated. LT50 values (°C) for clonal varieties were: GN-1 = -5.8, Baby = -6.1, Tifway = -6.6, Tifton 94, Quickstand = -8.0, and Midlawn = -8.4. LT50 values for seeded varieties were: Arizona Common = -5.6, Mirage = -6.1, Jackpot = -6.3, Guymon = -7.4, and OKS 91-11 = -7.6. These evaluations will continue with selected varieties from: 1) vegetatively-propagated fairway types, 2) seeded fairway types, 3) vegetatively-propagated putting green types, and 4) experimental fairway breeding lines. The primary structure of the preprotein encoded by the bermudagrass chitinase genes (CynCht1, CynCht2) were analyzed. Both chitinase genes encode low molecular weight hydrophilic (secreted) proteins, which can be structurally classified as Class II chitinase. The mature polypeptide of CynCht1 is composed of 227 amino acid residues with a molecularweight of 25 kDa and calculated pI of 8.10. CynCht2 mature polypeptide, on the other hand, consists of 229 with a molecular weight of 25.5 kDa and calculated pI of 8.82. Alignment of the amino acid sequences of the mature polypeptides encoded by the two genes revealed significant homology with a number of known chitinases from higher plants. Both chitinases are most closely related to the Class II chitinases from peanut and tomato. Functional analysis on the products of the low temperature-inducible CynCht1 gene via Agrobacterium - mediated transformation of Arabidopsis thaliana is underway. Selection of a suitable Arabidopsis ecotype has been conducted by determining the cold hardiness of 10 ecotypes collected from a variety of cold and warm habitats. Results suggested that the Tmid values (using electrolyte leakage testing) slightly differ between the non-acclimated and cold acclimated plants and there is no significant difference in the cold hardiness of the 10 ecotypes evaluated so far. The plasmid that will be used for Agrobacterium-mediated transformation (Via the binary system) is being constructed. The coding region (1.2 kb) of the CynCht1 from the main clone Stul 456-1 was PCR amplified using forward and reverse primers. Procedures for inserting the CynCht1 gene into the binary T-DNA vector (pBECKS), recently provided by Dr. Alex McCormac from the University of Southampton, UK, are being optimized." |
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| Language: | English |
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| References: | 29 |
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| See Also: | See also related summary article, "Bermudagrass cold hardiness: Characterization of plants for freeze tolerance and character of low temperature-induced genes", 2000 Turfgrass and Environmental Research Summary [USGA], 2000, p. 41, R=72143. R=72143 |
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| Note: | Figures
Tables
Graphs
"Annual Progress Report"
"For the period 1 November 1999 - 31 October 2000"
Also appears as pp. 453-468 in the USGA Turfgrass Research Committee Reporting Binders for 2000. |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
No defined citation format for TGIF #: 215163 |
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http://archive.lib.msu.edu/tic/rpr/2000/72143,%20OK%20State, Taliaferro, Cold Hardiness.PDF
Last checked: 02/11/2013
Requires: PDF Reader |
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