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| Web URL(s): | http://archive.lib.msu.edu/tic/rpr/1999/72310,%20Michigan%20State,%20Sticklen.PDF
Last checked: 02/25/2013
Requires: PDF Reader |
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| Publication Type:
| Report |
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| Material Type: | Manuscript |
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| Monographic Author(s): | Sticklen, Mariam; Vargas, Joseph; Baird, James |
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| Author Affiliation: | Sticklen: Associate Professor, Crop & Soil Sci. Dept.; Vargas: Professor, Bot. & Plant Pathol. Dept.; and Baird: Assistant Professor, Crop & Soil Sci. Dept., Michigan State University, East Lansing, MI |
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| Monograph Title: | A Multi-Gene Transfer Strategy to Control Pathogens and Enhance Environmental Stress Tolerance in Creeping Bentgrass: [1999] Annual Report to The United States Golf Association, 1999. |
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| Publishing Information: | East Lansing, Michigan: Michigan State University |
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| # of Pages: | 7 |
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| Collation: | 7 pp. |
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| Abstract/Contents: | "Major biotic and abiotic problems associated with the management of creeping bentgrass turf include: several pathogenic disorders and certain environmental extremes such as drought, heat, and cold stress. Environmental extremes such as drought can influence the health of the plant and its ability to resist infection by biotic agents. Resistance to biotic and abiotic stresses in plants has been reported to be associated with relatively complex genetic factors. Most biotechnological approaches of the last two decades, especially those related to the control of insects and diseases, have concentrated on transferring a single gene to plants. The single gene approach may sound attractive over a short period of time, however, this approach may result in more serious problems over longer periods of time as populations of biotic agents develop resistance to the single gene. Our long term goals include development of transgenic turfgrasses with improved resistance to pathogens and drought tolerance. Previously, Sticklen's research team developed creeping bentgrass clones that contain: a glufosinate ammonium (Liberty) herbicide resistance gene [1]; a chitinase gene [2]; a proteinase inhibitor gene [3]; and a mannitol dehydrogenase (mt1D) gene for drought and salt tolerance [4]. So far, our research team confirmed that glofosinate ammonium has fungicidal as well as herbicidal properties. Therefore, we have been able to simultaneously control turfgrass pathogens (mainly Sclerotinia ulnocarpal and Rhizoctonia solani) as well as weeds by spraying this herbicide on transgenic creeping bentgrass expressing the herbicide resistance gene under greenhouse conditions [2]. Studies have shown that chitinase genes can make transgenic plants resistant to pathogenic fungi such as R. solani. [5]. Research by Dr. Vargas' laboratory has shown that our transgenic creeping bentgrass clone 711, transcribing the elm chitinase gene controlled by the cauliflower mosaic virus 35S promoter, has improved resistance to R. solani under controlled environmental conditions. Dr. Sticklen's laboratory has constructed a plasmid containing the elm chitinase gene controlled by a rice actin promoter and transformed creeping bentgrass with this construct. Molecular analysis is in progress to test the expression of the chitinase gene regulated by this grass-specific promoter in creeping bentgrass. The mannitol 1-phosphate dehydrogenase gene for drought tolerance (mt1D) that we have used to transform creeping bentgrass [6; 2] is also associated with salt tolerance [7]. Experiments performed by Dr. Baird's laboratory have not shown any drought tolerance in transgenic plants, nor have they shown accumulation of mannitol in transgenic plants. Antibodies were made against the enzyme, mannitol dehydrogenase, using synthetic peptides. Western blotting will be performed to test the level of expression of this gene in different transgenic lines." |
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| Language: | English |
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| References: | 14 |
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| See Also: | See also related summary article, "A multigene-transfer strategy to improve disease and environmental stres resistance in creeping bentgrass", 1999 Turfgrass and Environmental Research Summary [USGA], 1999, p. 30, R=72310. R=72310 |
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| Note: | "Annual Report to The United States Golf Association"
"October 1999" |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
No defined citation format for TGIF #: 215858 |
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Last checked: 02/25/2013
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