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| Web URL(s): | http://archive.lib.msu.edu/tic/rpr/1998/61840,%20Michigan%20State,%20Sticklen.PDF
Last checked: 10/18/2013
Requires: PDF Reader |
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| Publication Type:
| Report |
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| Material Type: | Manuscript |
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| Monographic Author(s): | Sticklen, Mariam; Vargas, Joseph; Baird, James |
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| Author Affiliation: | Sticklen: Associate Professor, Crop & Soil Sci. Dept.; Vargas: Professor, Bot. & Plant Pathol. Dept.; and Baird: Assistant Professor, Crop & Soil Dept., Michigan State Uni., East Lansing, MI |
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| Monograph Title: | A Multigene-Transfer Strategy to Control Pathogens and Enhance Environmental Stress Tolerance in Creeping Bentgrass: [1998 Annual Research Report], 1998. |
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| Publishing Information: | East Lansing, Michigan: Michigan State University |
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| # of Pages: | 5 |
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| Collation: | 5 pp. |
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| Abstract/Contents: | "Major biotic and abiotic problems associated in the management of creeping bentgrass turf include several pathogenic disorders and certain environmental extremes such as drought, heat, and cold stress. In addition, environmental extremes such as drought can influence the health of the plant and its ability to resist infection by biotic agents. Resistance to biotic and abiotic stress in plants had been reported to be associated with relatively complex genetic factors. Most biotechnological approaches of the last two decades, specially those related to the control of insects and diseases, have concentrated on transferring a single gene to plants. The single gene approach may sound attractive over a short period, however, this approach may result in more serious problems over longer periods as populations of biotic agents develop resistance to the single gene increase. Our long term goals include development of transgenic turfgrasses with improve resistance to pathogens and drought tolerance. Previously, Sticklen's research team developed creeping bentgrass clones that contain (1) the glufosinate ammonia (Liberty) resistant herbicide [1], a chitinae gene [2], a proteinase inhibitor gene [3], and a drought and salt tolerance mannitol dehydrogenase (mt1D) gene [2]. So far, our research team confirmed that glufosinate ammonia has fungicidal as well as herbicidal properties. Therefore, we have been able to simultaneously control weeds as well as turfgrass pathogens (mainly Sclerotinia ulnocarpal and Rhizoctonia solani) by spaying this herbicide on transgenic creeping bentgrass expressing this gene under greenhouse conditions [4]. Studies have shown that the chitinase genes can make transgenic plants resistant to pathogenic fungi such as R. solani, etc. [5]. Research by Dr. Vargas laboratory has shown that our transgenic creeping bentgrass clone 711, transcribing the elm chitinase gene controlled by the cauliflower mosaic virus 35S promoter, has improved resistance of plants to R. solani under controlled environmental conditions. Recently, Dr. Sticklen laboratory has constructed a plasmid containing the elm chitinase gene controlled by rice actin promoter (shown to provide greater gene expression in grass family than the 35S promoter) and transformed creeping bentgrass with this construct. Theoretically, using this grass-specific promoter, we should improve the level of expression of the chitinase gene, and the degree of resistance to R. solani in transgenic creeping bentgrass plants. The mannitol 1-phosphate dehydrogenase (mt1D:known for its drought tolerance) gene that we have used to transform creeping bentgrass [6; 2] is also associated with salt tolerance [7],[.] A preliminary experiment performed by Dr. Baird laboratory has not shown any drought resistance tolerance of transgenic plants. More studies are needed to confirm whether these plants have any tolerance to drought and/or salt." |
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| Language: | English |
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| References: | 0 |
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| See Also: | See also related summary article "A multigene-transfer strategy to improve disease and environmental stress resistance in creeping bentgrass" 1998 Turfgrass and Environmental Research Summary [USGA], 1998, pp. 37-38, R=61840. R=61840 |
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| Note: | Also appears as pp. 176-180 in the USGA Turfgrass Research Committee Reporting Binders for 1998.
"Annual Report to The United States Golf Association"
"October 1998" |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
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Last checked: 10/18/2013
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