| |
| Web URL(s): | http://archive.lib.msu.edu/tic/rpr/1998/61845,%20NC%20State,%20Qu.PDF
Last checked: 10/18/2013
Requires: PDF Reader |
|---|
| Publication Type:
| Report |
|---|
| Material Type: | Manuscript |
|---|
| Monographic Author(s): | Qu, Rongda; Chaudhury, Ashok; Davis, Eric; Schubert, Karel |
|---|
| Author Affiliation: | Qu: PI, Department of Crop Science, North Carolina State University; Chaudhury: Postdoctoral Associate; Davis: Assistant Professor, Plant Pathology, NCSU; and Schubert: Professor, Botany, University of Oklahoma and Collaborator |
|---|
| Monograph Title: | Hybrid Bermudagrass Improvement By Genetic Transformation: [1998 Annual Research Report], 1998. |
|---|
| Publishing Information: | [Raleigh, North Carolina]: Department of Crop Science, North Carolina State University |
|---|
| # of Pages: | 6 |
|---|
| Collation: | 5, [1] pp. |
|---|
| Abstract/Contents: | "The ultimate goal of this research direction is to improve bermudagrass cultivars for the golf courses through biotechnology. The specific goals of the project include: to optimize tissue culture conditions for inducing embryonic calli and regenerating plantlets of bermudagrass; to develop procedure to transform the embryogenic calli by the biolistic method and to recover transgenic plants, and to obtain transgenic plants of bermudagrass that express potential nematode resistant genes. Bermudagrass is a recalcitrant species for plant tissue culture. Thus, so far most of the efforts have been concentrated on optimizing tissue culture conditions, especially at the callus induction stage to improve the callus quality and the regeneration ability. Various tissues, culture media and supplements to the media have been tested in order to optimize tissue culture conditions of bermudagrass. It was found that approximately 20% of the calli induced from young inflorescence (0.5 to 1 cm) of 'Tifgreen' and 'Savannah' (a common bermudagrass cultivar) had an embryonic structure when cultured is MS medium (1 mg/L 2,4-D) supplemented with 0.01 mg/L 6-benzylaminopurine (BAP). No such structure were found in media without BAP. The calli were slow-growing, compact, pale or off-white in color, and highly regenerable. The regeneration rate of the calli with embryonic structure was higher than 50% while calli without this structure had the regeneration rate about 1-5%. In addition, the callus induction rate was raised from 21-33% to over 60% by excising young inflorescence into pieces before the culture inoculation. It was very different to induce callus from young inflorescence of 'Tifway' due to the quick browning of the explants in culture medium. The situation can be improved by pretreatment of explants with 0.2% ascorbic acid, an anti-oxidant. Pilot experiments were performed to determine the pressure parameter of the biolistic apparatus. It was found by transient assay of GUS reporter gene that bombardment at 1550 psi on osmotically treated calli were the best for transformation experiment. Callus growth inhibited assays were performed with three potential selection agents at various levels. It was found that 250 mg/L was an effective selection level for both antibiotics, kanamycin and hygromycin B, while 5 mg/L was appropriate for selection with bialaphos, an herbicide." |
|---|
| Language: | English |
|---|
| References: | 0 |
|---|
| See Also: | See also related summary article "Hybrid bermudagrass improvement by genetic transformation" 1998 Turfgrass and Environmental Research Summary [USGA], 1998, p. 41, R=61845. R=61845 |
|---|
| Note: | Also appears as pp. 184-189 in the USGA Turfgrass Research Committee Reporting Binders for 1998.
"1998 Annual Report to United States Golf Association"
"November 1, 1998"
Pictures, b/w
Tables |
|---|
| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
No defined citation format for TGIF #: 231443 |
| Fastlink to access this record outside TGIF: https://tic.lib.msu.edu/tgif/flink?recno=231443 |
| If there are problems with this record, send us feedback about record 231443. |
| Choices for finding the above item: |
|---|
| Web URL(s):
http://archive.lib.msu.edu/tic/rpr/1998/61845,%20NC%20State,%20Qu.PDF
Last checked: 10/18/2013
Requires: PDF Reader |
| Find from within TIC:
Digitally in TIC by record number. |
| Request through your local library's inter-library loan service (bring or send a copy of this TGIF record) |
