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| Web URL(s): | http://archive.lib.msu.edu/tic/rpr/1997/54563%20,%20Michigan%20State,%20Sticklen.PDF
Last checked: 10/24/2013
Requires: PDF Reader |
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| Publication Type:
| Report |
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| Material Type: | Manuscript |
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| Monographic Author(s): | Vargas, Joseph; Sticklen, Mariam; Liu, Chien-An; Chai, Benli |
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| Author Affiliation: | Vargas: Professor, Bot. & Plant Pathol. Dept.; Sticklen: Associate Professor, CSS Dept. Michigan State University, East Lansing, MI; Liu: Ph.D.; and Chai: Ph.D. Student |
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| Monograph Title: | [Genetic Engineering of Creeping Bentgrass With Chitinase and a Biolophosherbicide Resistance Genes: 1997 Final Research Report], [1997]. |
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| Publishing Information: | East Lansing, Michigan: Michigan State University |
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| # of Pages: | 13 |
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| Collation: | 12, [1] pp. |
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| Abstract/Contents: | "Dollar spot (Sclerotinia homeocarpa), brown patch (Rhizoctonia solani) and Pythium blight (Pythium aphanidermatum) are major pathogenic diseases of turfgrass.. Also, all of these pathogens contain chitin. The Sticklen laboratory team has cloned and characterized a full length chitinase gene which contains the necessary chitin-binding domain ([1; 2; 3] Gene Bank Number L22032). This laboratory also has constructed several plasmids containing a potato proteinase inhibitor II controlled by different (would-inducible, and constitutive) promoters. We have also obtained genes for a drought resistance and the bialaphos resistance from other laboratories. During the last three years, an enormous amount of research was performed as follows. We genetically engineered tobacco as a model system and then creeping bentgrass with the chitinase gene construct that was developed in Sticklen's laboratory. We also engineered creeping bentgrass with a few other useful genes including protease [proteinase] inhibitor II, the bar (bialaphos resistance), and the mannitol dehydrogenase (drought tolerance) genes. The herbicide resistance of these transgenic plants was confirmed over two years ago. These plants have been transferred to our field facilities, as well as to Bill Rose's field facilities. We examined the chitinase-transgenic plants containing the chitinase gene for their resistance to brown patch, and bar-transgenic plants after they were sprayed with bialaphos for dollar spot, brown patch, and Pythium diseases. Preliminary work on chitinase-transgenic plants at the greenhouse level showed that these plants are tolerant to brown patch disease. Furthermore. the results of our inoculation studies have shown that after we spray on the bar-transgenic creeping bentgrass, we can simultaneously control weeds, dollar spot and brown patch diseases at the greenhouse level. Eventually, all these transgenic plants must be crossbred and tested for their resistance to the major pathogenic diseases at the field level." |
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| Language: | English |
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| References: | 0 |
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| See Also: | See also related summary article "Development of transgenic creeping bentgrass resistant to the major pathogenic fungi" 1997 Turfgrass and Environmental Research Summary [USGA], 1997, p. 37-38, R=54563. R=54563 |
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| Note: | Also appears as pp. 427-440 in the USGA Turfgrass Research Committee Reporting Binders for 1997.
"Final Report to The United States Golf Association"
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Last checked: 10/24/2013
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