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| Web URL(s): | http://archive.lib.msu.edu/tic/rpr/1996/Turfgrass/46966,%20Michigan%20State,%20Sticklen.PDF
Last checked: 11/06/2013
Requires: PDF Reader |
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| Publication Type:
| Report |
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| Material Type: | Manuscript |
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| Monographic Author(s): | Vargas, Joseph; Sticklen, Mariam |
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| Author Affiliation: | Vargas: Professor, Bot. & Plant Pathol. Dept.; and Sticklen: Associate Professor, CSS Dept., Michigan State University, East Lansing, MI |
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| Monograph Title: | Genetic Engineering of Creeping Bentgrass With Chitinase and a Bialaphos-Herbicide Resistance Genes: [1996 Annual Research Report], [1996]. |
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| Publishing Information: | East Lansing, Michigan: Michigan State University |
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| # of Pages: | 10 |
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| Collation: | 10 pp. |
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| Abstract/Contents: | "The major problem associated with creeping bentgrass are pathogenic diseases. Most pathogens contain chitin in their cell walls, and therefore may be susceptible to chitinases [1]. The objective of this project was to develop fungal diseases resistant creeping bentgrass/Penncross (Agrostis palustris Huds.) plants, initially by introducing a chitinase gene in this plant. However, after being funded we realized that one single gene may not necessarily work, and pathogens may develop resistance against a single gene product within a short period of time. Therefore, we supplemented four fold research to our proposed project. These research lines include (1) introducing a chitinase gene as proposed, (2) introducing a protease inhibitor gene in plants (because proteases which are essential for the survival of the pathogenic fungi), (3) introducing a drought resistance gene in plants (because less need for irrigation would prevent growth and spread of diseases), and (4) introducing a bialaphos resistance gene to creeping bentgrass (because should plants become resistant to bialaphos-herbicide, bialaphos would simultaneously control weeds as well as certain pathogenic diseases). During last year, we followed up on introducing a chitinase gene, a protease inhibitor gene, the bar (bialaphos resistance) gene, and a drought resistance (mannitol dehydrogenase) gene into creeping bentgrass. As we reported earlier, we previously developed transgenic creeping bentgrass which express a potato proteinase gene and the bialaphos-herbicide resistance genes. At present, these transgenic plants are in the field. The herbicide resistance of these transgenic plants were confirmed a long time ago. Within last year, we examined these transgenic plants after they were sprayed with biolophos for their resistance to dollar spot, brown patch and Pythium. The results confirmed that after we spray transgenic creeping bentgrass with biolophos (Liberty herbicide), we simultaneously control weeds, dollar spot and brown patch diseases at the greenhouse level. This experiment will be repeated at the field level by Dr. Vargas next summer. We have also introduced the chitinase gene and a drought resistance gene to creeping bentgrass. The selectable marker for this experiment has also been the bialaphos resistance gene. All plants regenerated are bialaphos resistance. Since in our construct, the herbicide resistance gene was linked to the drought resistance gene, we are sure that these transgenic plants also contain the drought resistance gene. Hundreds of transgenic plants have been produced from the chitinase/drought resistance experiment. Work is in progress to confirm the stable integration and expression of the chitinase gene and the drought resistance gene in these plants. Eventually, all these transgenic plants must be cross bred and tested for their resistance to the major pathogenic diseases." |
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| Language: | English |
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| References: | 12 |
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| See Also: | See also related summary article "Development of transgenic creeping bentgrass resistant to the major pathogenic fungi" 1996 Turfgrass and Environmental Research Summary, 1996, p. 42-43, R=46966. R=46966 |
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| Note: | Also appears as pp. 00457-00467 in the USGA Turfgrass Research Committe Reporting Binders for 1996. |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
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| Web URL(s):
http://archive.lib.msu.edu/tic/rpr/1996/Turfgrass/46966,%20Michigan%20State,%20Sticklen.PDF
Last checked: 11/06/2013
Requires: PDF Reader |
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