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| Web URL(s): | http://archive.lib.msu.edu/tic/rpr/1995/Turfgrass/21317,%20Rutgers, Day.PDF
Last checked: 11/11/2013
Requires: PDF Reader |
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| Publication Type:
| Report |
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| Material Type: | Manuscript |
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| Monographic Author(s): | Day, Peter |
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| Monograph Title: | Development of Improved Turfgrass With Herbicide Resistance and Enhanced Disease Resistance Through Transformation: [1995 Annual Research Report], 1995. |
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| Publishing Information: | [New Brunswick, New Jersey]: [Rutgers University] |
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| # of Pages: | 14 |
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| Collation: | 14 pp. |
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| Abstract/Contents: | "This project seeks to improve creeping bentgrass through transformation to provide golf course managers with more effective and selective weed control with herbicides and more environmentally sound and cost-effective control of plant diseases with reduced use of fungicides. We have accomplished several major goals: creeping bentgrass tissue culture and regeneration system; successful biolistic and protoplast transformations of creeping bentgrass; recovery of several cultivars of creeping bentgrass with resistance to two different herbicides; field tests of clones of all herbicide-resistant creeping bentgrass; turf quality (including disease resistance) study of herbicide-resistant transgenic creeping bentgrass; a progeny analysis of Finale-resistant creeping bentgrass is underway. We are making good progress in incorporating single gene traits for herbicide resistance and enhanced disease resistance in turfgrass. We have expanded the creeping bentgrass tissue culture and regeneration system from nine creeping bentgrass cultivars to several other elite cultivars including 'Penncross', 'Penneagle', 'Crenshaw', and 'A-1'. Transgenic creeping bentgrass clones of 'Cobra', 'Emerald', and 'Southshore' have been obtained from particle and protoplast transformation with resistance to herbicide bialaphos. They are resistant to 5x the field rate in greenhouse herbicide spray tests and up to 3x the recommended rate in field herbicide applications of FinaleTM. Field tests of herbicide-resistant creeping bentgrass were conducted in 1994 and 1995. Transgenic creeping bentgrasses from the 1994 field test were vernalized in the field overwinter and returned to a containment greenhouse in the spring before flowering. At flowering they were cross-pollinated with wild type plants. Seeds were harvested and progeny analyses to determine heritability of the transgene will be performed in the fall of 1995. This will be the first study of transgene transmission in creeping bentgrass. Four field tests of herbicide-resistant creeping bentgrasses were conducted in 1995. The Cobra transgenic plants obtained from protoplast transformation showed a high level of herbicide tolerance, up to 3x the recommended field rate of Finale, like the transgenic plants tested in the 1994 field test. To enhance fungal disease resistance, we have performed greenhouse herbicide tests with putative transgenic plants that carry genes expressing bean chitinase, tobacco chitinase B and Maize chitinase, and have started testing transgenic plants for resistance against Rhizoctonia solani." |
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| Language: | English |
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| References: | 0 |
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| See Also: | See also related summary article "Development of improved turfgrass with herbicide resistance and disease resistance through transformation" 1995 Turfgrass and Environmental Research Summary, 1995, p. 46-47, R=21317. R=21317 |
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| Note: | "November 1995"
Tables |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
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http://archive.lib.msu.edu/tic/rpr/1995/Turfgrass/21317,%20Rutgers, Day.PDF
Last checked: 11/11/2013
Requires: PDF Reader |
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