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| Web URL(s): | http://archive.lib.msu.edu/tic/rpr/1995/Turfgrass/36346%20,%20U%20Florida-Giblin-Davis.PDF
Last checked: 11/12/2013
Requires: PDF Reader |
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| Publication Type:
| Report |
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| Material Type: | Manuscript |
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| Monographic Author(s): | Giblin-Davis, Robin M.; Dickson, Don W.; Cisar, John L. |
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| Author Affiliation: | Giblin-Davis and Cisar: Ft. Lauderdale Research and Education Center, University of Florida, Ft. Lauderdale, FL; and Giblin-Davis and Dickson: Department of Entomology and Nematology, University of Florida, Gainesville, FL |
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| Monograph Title: | Pasteuria sp. for Biological Control of the Sting Nematode, Belonolaimus longicaudatus, in Turfgrass: [1995 Annual Research Report], 1995. |
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| Publishing Information: | Gainesville, Florida: University of Florida |
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| # of Pages: | 21 |
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| Collation: | 5, [16] pp. |
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| Abstract/Contents: | "We are describing a new species of bacterium in the genus, Pasteuria that we discovered parasitizing the sting nematode, Belonolaimus longicaudatus in Florida. We are hopeful that this obligate bacterial parasite of nematodes (Pasteuria n. sp. [S-1]) will have some potential for inoculative biological control in golf course greens against the sting nematode; a destructive ectoparasite that can reduce the root dry weight of turfgrasses and other crops in sandy soils by as much as 30-50%. In 1995, we completed ultrastructural studies with transmission electron microscopy (TEM) and low-temperature scanning electron microscopy (SEM) that show that Pasteuria n. sp. (S-1) is a new species. These studies have also helped to finish elucidating the development and life cycle of this bacterium. We now have excellent photomicrographs illustrating all aspects of the biology of Pasteuria n. sp. (S-1). Use of the new technique of low temperature SEM has helped to visualize spore morphology outside and inside the infected nematodes without the usual artifacts associated with TEM. We have now isolated another population of this bacterium from a golf course in Gainesville, FL and we are doing TEM to confirm that it is ultrastructurally similar to Pasteuria n. sp. (S-1). This will give us a better idea of the possible distribution of this bacterium within Florida. A population dynamic study (390 days long) was completed on Pasteuria n. sp. (S-1) in laboratory pot cultures of the sting nematode on the model turfgrass host (FX-313 St. Augustinegrass) under controlled conditions. There were four treatments; 1) no sting nematodes with no bacteria, 2) sting nematodes (99 ± 10) with no bacteria, 3) sting nematodes (99 ± 10) + 10 sting nematodes encumbered with 8 ± 6 spores of Pasteuria n. sp. (S-1, and 4) sting nematodes (99 ± 10) + 25 sting nematodes encumbered with 8 ± 6 spores of Pasteuria n. sp. (S-1). The assumption was that inoculated sick nematodes would not add to the population growth of the healthy sting nematodes but would die and release bacteria that would negatively affect the healthy population. Our results demonstrate that this was not the case. Population dynamics of the healthy sting nematodes were increased by the addition of the "sick" nematodes suggesting that spore encumbrance is not a good indicator of spore production or nematode health. Root dry weights for the different treatments confirmed that the greatest root loss occurred in the treatments with the most nematodes. Although spore encumbered sting nematodes were recovered throughout most of the 390 day study the levels were never greater than 1% from treatments receiving spores which suggests that inoculative release of "sick" nematodes is unacceptable for establishment and population suppression work. In 1995, we also began a monthly survey of 6 different sites of hybrid bermudagrass (fairway conditions) at the Ft. Lauderdale Research and Education Center where Pasteuria n. sp. (S-1) occurs naturally at different levels to monitor its suppressive effects at three different soil depths on sting nematodes. Soil temperature was also monitored at these different depths. After 6 months of sampling, locations that started with low levels of spore encumberance [encumbrance] had higher numbers of sting nematodes than areas that started with high encumberance [encumbrance] levels, suggesting that Pasteuria n. sp. (S-1) might help produce suppressive soil for the sting nematode. These results are encouraging but will require at least one year of survey work." |
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| Language: | English |
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| References: | 0 |
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| See Also: | See also related summary article "Pasteuria sp. for biological control of the sting nematode (Belonolaimus longicaudatus), in turfgrass" 1995 Turfgrass and Environmental Research Summary, 1995, pp. 60-61, R=36346. R=36346 |
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| Note: | "1995 USGA Turfgrass Research Report"
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Last checked: 11/12/2013
Requires: PDF Reader |
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