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| Web URL(s): | http://archive.lib.msu.edu/tic/rpr/1995/Turfgrass/3766,%20Virginia%20State,%20Ha.PDF
Last checked: 11/12/2013
Requires: PDF Reader |
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| Publication Type:
| Report |
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| Material Type: | Manuscript |
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| Monographic Author(s): | Ha, Sam B. |
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| Author Affiliation: | Virginia Polytechnic Institute and State University |
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| Monograph Title: | Development of Genetically Engineered Creeping Bentgrass Resistant to Fungal Diseases: [1995 Annual Research Report], [1995]. |
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| Publishing Information: | [Blacksburg, Virginia]: Virginia Polytechnic Institute and State University |
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| # of Pages: | 11 |
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| Collation: | [1], 7, [3] pp. |
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| Abstract/Contents: | "This project is designed to improve disease resistance of creeping bentgrass using genetic engineering. The main objectives are 1) to develop a gene transfer system in creeping bentgrass and 2) to development genetically engineered creeping bentgrass with overexpression of chitinase genes. We have developed an efficient gene delivery system for creeping bentgrass using particle bombardment and selecting transformed calli with hygromycin. We have also isolated three genomic clones of chitinase genes (chi1, chi2, chi3) from Kentucky bluegrass using adaptor-ligation polymerase chain reaction (PCR). chi1 and chi2 encode full length chitinases of 340 and 320 amino acids, respectively. chi3 appears to encode a truncated chitinase (49 amino acids) due to the presence of a stop codon in the coding region. Using reverse transcription and PCR, we found that both chi1 and chi2 were induced by ethylene, strongly indicating both genes were involved in plant defense responses. We subcloned each of these two genes (chi1 and chi2) into the expression vector and transferred the plasmids containing the Kentucky bluegrass chitinase genes to creeping bentgrass by particle bombardment. Transformed calli have been selected on the medium containing hygromycin and we are currently regenerating transgenic plant from these calli. Once transgenic plants are developed, those which exhibit a high level of chitinase expression will be screened and tested for the resistance to fungal pathogens." |
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| Language: | English |
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| References: | 0 |
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| See Also: | See also related summary article "Development of genetically engineered creeping bentgrass resistant to fungal diseases" 1995 Turfgrass and Environmental Research Summary [USGA], 1995, pp. 44-45, R=3766. R=3766 |
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| Note: | "Third Year Progress Report"
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| Web URL(s):
http://archive.lib.msu.edu/tic/rpr/1995/Turfgrass/3766,%20Virginia%20State,%20Ha.PDF
Last checked: 11/12/2013
Requires: PDF Reader |
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