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| Web URL(s): | http://archive.lib.msu.edu/tic/rpr/1995/Turfgrass/8968,%20Mississippi State, Krans.PDF
Last checked: 11/12/2013
Requires: PDF Reader |
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| Publication Type:
| Report |
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| Material Type: | Manuscript |
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| Monographic Author(s): | Tomaso-Peterson, Maria; Krans, J. V. |
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| Author Affiliation: | Mississippi State University |
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| Monograph Title: | Recovery of Rhizoctonia solani Resistant Creeping Bentgrass Germplasm Using the Host-Pathogen Interaction System: [1995 Final Research Report], 1995. |
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| Publishing Information: | [Mississippi State, Mississippi]: Mississippi State University |
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| # of Pages: | 20 |
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| Collation: | [20] pp. |
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| Abstract/Contents: | "The Host-Pathogen Interaction System (HPIS) is an in vitro cell selection system developed in conjunction with efforts to obtain creeping bentgrass with resistance to Rhizoctonia solani. The primary objective throughout our USGA research projects has been to verify HPIS as a valid in vitro cell selection system. With this objective achieved, we can recover bentgrass germplasm from HPIS selections and evaluate those genotypes in the field with confidence that some will segregate from the population, exhibiting enhanced disease resistance. The first step in achieving our primary objective was to obtain disease resistant bentgrass callus via HPIS selection. HPIS refinement studies associated with our initial USGA research project confirmed selection of resistant callus. Callus mortality increased significantly as 'Penncross' calli co-cultured in HPIS with a virulent isolate of R. solani. Small numbers of plantlets were regenerated from resistant callus compared to high numbers of plantlets recovered from control populations. With bentgrass germplasm successfully regenerated from resistant calli, our USGA research project progressed from there to determine whether enhanced resistance could be exhibited at plantlet and whole plant levels. Plantlets were evaluated for tolerance to R. solani using two in vitro screening techniques: 1.) HPIS Chamber - Plantlets were placed in an HPIS Chamber, exposing them to R. solani for two weeks. Over 33% of the plantlets did not survive. 2.) Leaf Bioassy - Leaves of plantlets were exposed to exudate produced by R. solani. Plantlets recovered from resistant callus displayed significantly less leaf necrosis as compared to control plantlets. Plantlets surviving both screening techniques were subsequently transferred to soil (whole plant) and maintained in a greenhouse. Selected bentgrass plants [variant(s)] were inoculated with R. solani using growth chamber techniques and evaluated for disease response. Preliminary results indicated some variants expressed enhanced resistance to R. solani. Based on these findings, variants were established in the field under putting green conditions. Establishment and turf quality data indicate the majority of variants are similar to or better than Penncross. Concurrent with establishment, variants were rated for brown patch resistance based on natural infestation. Preliminary observations indicate enhanced resistance may exist among some variants. Several plots displayed brown patch symptoms while adjacent plots had no symptomology. Bentgrass variants will be evaluated under various stress and environmental conditions through two successive brown patch seasons (2 yr). Natural infestation and field inoculations will occur under natural putting green conditions. Results from these evaluations will provide us the opportunity to confidently identify variants exhibiting enhanced resistance to brown patch. Selected variants will be used to improve existing creeping bentgrass gene pools by incorporating genes with enhanced R. solani resistance. A clonal repository of HPIS germplasm is maintained in an environment controlled greenhouse at Mississippi State University. To date, almost 200 genotypes are established under putting green conditions at locations in Mississippi and North Carolina. HPIS refinements studies have confirmed HPIS as a valid in vitro cell selection tool, giving us confidence that some bentgrass variants will be identified as having enhanced resistance brown patch." |
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| Language: | English |
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| References: | 13 |
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| See Also: | See also related summary article "Recovery of Rhizoctonia solani resistant creeping bentgrass using the host-pathogen interaction system" 1995 Turfgrass and Environmental Research Summary, 1995, p. 42-43, R=8968. R=8968 |
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| Note: | "Final Research Progress Report 1995"
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http://archive.lib.msu.edu/tic/rpr/1995/Turfgrass/8968,%20Mississippi State, Krans.PDF
Last checked: 11/12/2013
Requires: PDF Reader |
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