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| Web URL(s): | http://archive.lib.msu.edu/tic/rpr/1994/35215,%20Clemson,%20Baird.PDF
Last checked: 11/14/2013
Requires: PDF Reader |
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| Publication Type:
| Report |
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| Material Type: | Manuscript |
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| Monographic Author(s): | Baird, Wm. Vance; Miller, Landon C. |
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| Author Affiliation: | Investigator, Horticulture Department, Clemson University |
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| Monograph Title: | Low Temperature and Drought Regulated Gene Expression in Bermudagrass: [1994 Progress Report], 1994. |
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| Publishing Information: | [Clemson, South Carolina]: Clemson University |
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| # of Pages: | 12 |
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| Collation: | [12] pp. |
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| Abstract/Contents: | "Cellular membranes have been considered a primary site of freezing injury, and alterations of membrane composition correlate with cold acclimation processes that allow plants to tolerate freezing temperatures. As major components in membrane bilayers, the polar lipid fatty acids could directly regulate membrane structure, and therefore membrane function, through the alterations of acyl chain length (number of carbon atoms) and/or unsaturation (number of double bonds). Alterations in plant membrane lipid fatty acids can be induced by many physiological and environmental factors, and these changes could play an important role in adaption to low temperature. Bermudagrass (Cynodon dactylon) shows an increased tolerance to cold after a period of exposure to moderately low temperatures. However, whether this cold acclimation correlates with cell membrane alterations, and how the membrane lipid fatty acids (MLFA) respond to low temperature are unknown for bermudagrass. Bermudagrass total MLFA (ug), per unit of total lipids (mg), increased in crown tissues, but not roots or leaves, over the 4-week exposure to moderately low temperatures (8°C d/ 3°C n, 14 hr photoperiod). The major fatty acids in bermudagrass were determined to be palmitic acid (C18:3). These four made up 95% of the total MLFA. In crown tissues the concentration of shorter chain and saturated fatty acids (e.g., C16:0, C18:0) declined significantly during the cold treatment, while the concentration of the longer chain, unsaturated fatty acids (e.g., C18:3) increased. As a result, the double bond index increased in crown tissues over this same four week period. These changes increase the fluidity of membranes, and therefore, could reduce cold-induced membrane leakage and freezing injury. mRNA profiling/differential display techniques are being refined and employed in our efforts to characterize genetic polymorphisms between bermudagrass cultivars differing in levels of cold tolerance. These PCR based methods allow for the relatively rapid identification and cloning of gene sequences differentially expressed in response to a particular environmental stimulus such as low temperature, drought or chemical applications. The reproducibility of this technique and the identification of the appropriate oligonucleotide primers has been the focus of our current efforts." |
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| Language: | English |
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| References: | 0 |
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| See Also: | See also related summary article, "Low temperature and drought regulated gene expression in bermudagrass", 1994 Turfgrass Research Summary [USGA], 1994, p. 32-33, R=35215. R=35215 |
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| Note: | Also appears as pp. 00303-00314 in the USGA Turfgrass Research Committee Reporting Binders for 1994
"May 1, 1994 to October 31, 1994"
Graphs |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
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