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| Web URL(s): | http://archive.lib.msu.edu/tic/rpr/1994/35246,%20Rutgers,%20Day.PDF
Last checked: 11/14/2013
Requires: PDF Reader |
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| Publication Type:
| Report |
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| Material Type: | Manuscript |
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| Monographic Author(s): | [Day, Peter] |
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| Monograph Title: | Development of Improved Turfgrass with Herbicide Resistance and Enhanced Disease Resistance Through Transformation: [1994 Annual Research Report], 1994. |
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| Publishing Information: | [New Brunswick, New Jersey: Rutgers University] |
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| # of Pages: | 12 |
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| Collation: | 12 pp. |
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| Abstract/Contents: | "This project seeks to improve creeping bentgrass through transformation to provide golf course managers with more effective and selective weed control with herbicides and more environmentally sound and cost-effective control of plant diseases with reduced use of fungicides. We have reached several milestones; successful turfgrass transformation, efficient tissue culture and regeneration systems, recovery of several cultivars of creeping bentgrass with resistance to two different herbicides, and field tests of clones of Ignite-resistant creeping bentgrass. Transgenic bentgrass, a product of laboratory experimentation, shows promise as a useful tool for golf course management. We are making good progress in incorporating single gene traits for herbicide resistance and enhanced disease resistance in turfgrass. We now have embryogenic callus lines and suspension cultures derived from them with high regeneration potential from nine creeping bentgrass cultivars. We have established both particle gun and protoplast transformation systems for creeping bentgrass and have obtained first generation stable transformants with resistance to the herbicide bialaphos. In tests of more than one thousand regenerants from transformed tissues for herbicide sensitivity in greenhouse, we have obtained over one hundred herbicide-resistant transgenic plants of 'Cobra', 'Emerald', and 'Southshore'. This summer, we conducted the first field test of herbicide-resistant creeping bentgrass in the USA, and showed that the transgenic plants were resistant to up to 3x (2.25 lb Al/A) the label rate (1.5 to four fluid ounces per gallon of water). To enhance fungal disease resistance in turfgrass, three chitinase gene constructs were obtained, adapted to our transformation vectors, and introduced into creeping bentgrass through particle gun and protoplast transformation. We have begun greenhouse herbicide tests with putative transgenic plants that carry genes expressing bean chitinase or tobacco chitinase B, and are preparing to test transgenic plants for disease resistance." |
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| Language: | English |
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| References: | 0 |
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| See Also: | See also related summary article, "Development of improved turfgrass with herbicide resistance and enhanced disease resistance through transformation", 1994 Turfgrass Research Summary [USGA], 1994, p. 38, R=35246. R=35246 |
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| Note: | "November 1994"
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Last checked: 11/14/2013
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