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| Web URL(s): | http://archive.lib.msu.edu/tic/rpr/1994/35249,%20Virginia%20State,%20Ha.PDF
Last checked: 11/14/2013
Requires: PDF Reader |
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| Publication Type:
| Report |
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| Material Type: | Manuscript |
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| Monographic Author(s): | Ha, Sam B. |
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| Author Affiliation: | Virginia Polytechnic Institute and State University |
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| Monograph Title: | Development of Genetically Engineered Creeping Bentgrass Resistant to Fungal Diseases: [1994 Annual Progress Report], [1994]. |
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| Publishing Information: | [Blacksburg, Virginia]: Virginia Polytechnic Institute and State University |
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| # of Pages: | 13 |
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| Collation: | [1], 7, [5] pp. |
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| Abstract/Contents: | "This project is designed to improve disease resistance of creeping bentgrass via genetic engineering. The objectives are 1) to develop efficient gene transfer systems in creeping bentgrass and 2) to develop transgenic creeping bentgrass resistant to fungal diseases by overexpression of a chitinase gene. Chitinase is one of anti-fungal proteins produced in plants upon fungal infection. This enzyme catalyzes the hydrolysis of chitin, a cell wall component of many fungal pathogens. It was shown that constitutive overexpression of the chitinase gene in transgenic tobacco plants resulted in enhanced resistance to fungal diseases. For the second year of this project we focused our research efforts on developing a gene transfer system for creeping bentgrass and isolating a chitinase gene from Kentucky bluegrass. We have developed an efficient gene transfer system for creeping bentgrass using particle bombardment. A hygromycin resistance gene was transferred into embryogenic creeping bentgrass cells by particle bombardment and transformed cells were selected on the medium containing 150 or 200 mg/l hygromycin. A total of 124 transformed calli were obtained from 27 bombarded plates, with an average of 4.6 hygromycin-resistant colonies per bombardment. Thirteen transgenic plants were regenerated from the resistant colonies. Southern blot analysis confirmed the integration of the transgene into the genome of the transgenic plants. Using a PCR in vitro cloning method, we have isolated a chitinase gene from Kentucky bluegrass. We are currently determining the sequence of this gene." |
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| Language: | English |
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| References: | 0 |
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| See Also: | See also related summary article, "Development of genetically engineered creeping bentgrass resistant to fungal diseases", 1994 Turfgrass Research Summary [USGA], 1994, p. 42, R=35249. R=35249 |
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| Note: | "Second Year Progress Report"
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Last checked: 11/14/2013
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