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| Web URL(s): | http://archive.lib.msu.edu/tic/rpr/1994/35283,%20Cornell,%20Nelson.PDF
Last checked: 11/14/2013
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| Publication Type:
| Report |
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| Material Type: | Manuscript |
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| Monographic Author(s): | Nelson, Eric B.; Maloney, Alan P. |
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| Author Affiliation: | Nelson: Principal Investigator, Associate Professor; Maloney: Principal Investigator, Research Associate, Department of Plant Pathology, Cornell University, Ithaca, NY |
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| Monograph Title: | Genetic Basis of Biological Control in a Bacterium Antagonistic to Turfgrass Pathogens: [1994 Annual Research Report], 1994. |
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| Publishing Information: | Ithaca, New York: Department of Plant Pathology, Cornell University |
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| # of Pages: | 11 |
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| Collation: | [1], 10 pp. |
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| Abstract/Contents: | "The purpose of this project on E. cloacae genetics is to determine the array of bacterial traits responsible for biological control activity in bacteria. Our focus has been on Pythium-incited diseases of creeping bentgrass, but we believe our studies will have broad applicability to other bacterium-pathogen interactions. The objectives of our studies are to: 1)identify and clone DNA sequences that encode pathogen-suppressive properties in E. cloacae strain EcCT-501, 2) determine the nucleotide sequence of E. cloacae DNA encoding pathogen-suppressive properties and tentatively establish a function for the gene product, and 3) evaluate, in field studies, the expression of the biocontrol-related gene, psp1, under typical turfgrass management conditions. Our studies in 1994 focussed [focused] primarily on objective 2. Prior to the initiation of the work, we had isolated mutant V58 which was unable to suppress Pythium ultimum seed rot of cucumber. We were able to verify that whereas the wild-type strain, EcCT-501, was an effective biological control agent of Pythium graminicola on creeping bentgrass, mutant V58 was an ineffective biological control agent of against this pathogen. Our work subsequently was focussed [focused] on the molecular and physiological characterization of mutant V58. From complementational analysis, we have demonstrated that by rescuing the disrupted gene from wild-type cosmid library and mobilizing it into the mutant strain V58, we could effectively restore all of the biological phenotype of the wild-type strain. Using conventional molecular techniques, we have been able to isolate and sequence portions of the disrupted gene in mutant V58. From sequence analyses, we have discovered that the gene shares high homologies, both at the nucleotide and amino acid levels, with malate dehydrogenase from both E. coli and Samonella typhymurium. We performed a series of enzyme assays to verify that the gene we had cloned was actually a malate dehydrogenase (Mdh) gene. Clearly, the wild-type EcCT-501 possessed high levels of Mdh activity. This activity was totally absent in mutant V58. Furthermore, upon complementation with the putative mdh gene, Mdh activity was restored. Therefore, we feel confident that we have discovered a malate dehydrogenase gene with a major influence on the biological control of P. graminicola on creeping bentgrass and P. ultimum on cucumber." |
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| Language: | English |
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| References: | 3 |
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| See Also: | See also related summary article, "Genetic basis of biological control in a bacterium antagonistic to turfgrass pathogens", 1994 Turfgrass Research Summary [USGA], 1994, pp. 46-47, R=35283. R=35283 |
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| Note: | Also appears as pp. 00449-00459 in the USGA Turfgrass Research Committee Reporting Binders for 1994.
"United States Golf Association Green Section Research Proposal"
"October, 1994"
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