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| Web URL(s): | http://archive.lib.msu.edu/tic/rpr/1993/Turfgrass/65327,%20Rutgers,%20Day.PDF
Last checked: 11/20/2013
Requires: PDF Reader |
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| Publication Type:
| Report |
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| Material Type: | Manuscript |
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| Monographic Author(s): | [Day, Peter] |
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| Monograph Title: | Development of Improved Turfgrass With Herbicide Resistance and Enhanced Disease Resistance Through Transformation: [1993 Annual Research Report], 1993. |
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| Publishing Information: | [New Brunswick, New Jersey]: [Rutgers University] |
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| # of Pages: | 16 |
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| Collation: | 6, [10] pp. |
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| Abstract/Contents: | "This program aims to establish a transformation system for creeping bentgrass (Agrostis palustris) which will enable us to improve the utility of this important recreational turfgrass by incorporating genes to confer herbicide resistance or enhanced resistance to fungal pathogens. The improvements will help conserve natural resources by reducing chemical treatments against major fungal pests while providing high quality playing surfaces for golf through the ability to remove weeds and competing species using available, broad-spectrum and safe herbicides. Milestones for successful turfgrass transformation are: (1) establishment of a plant regeneration system to produce regenerants at high efficiency, (2) development of a high frequency transformation system to obtain large numbers of transgenic plants. Using surface sterilized mature seeds as explants for callus initiation, we developed a turfgrass tissue culture and regeneration system. Embryogenic callus lines with high regeneration potentials were established from eight commercially important creeping bentgrass varieties. Suspension cultures were initiated from embryogenic callus lines. Both embryogenic callus cultures and suspension cells were used as targets for stable transformation using a gene gun. The E.coli β-glucuronidase (GUS) gene was used as a scorable maker [marker] and the bar gene, which confers resistance to the herbicide bialaphos (BastaTM), was used as a selectable marker. We optimized various parameters to improve transient expression of GUS in cultured bentgrass to high levels, and we have developed a turfgrass transformation system with particle bombardment. Transformants were obtained from Emerald, Putter, and Southshore creeping bentgrass. Experiments incorporating several herbicide resistance genes in other cultivars are in various stages of completion. Both plate and liquid selection were successful in obtaining herbicide resistant bentgrass. Transgenics have been confirmed by herbicide tests, and by polymerase chain reaction (PCR) assay and southern blot hybridization to show the presence of the transgenes. We are also developing a protoplast transformation system. Regenerants were obtained from protoplasts through direct DNA uptake with polyethylene glycol (PEG) or electroporation. These plants will be tested for herbicide sensitivity by spraying in the greenhouse." |
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| Language: | English |
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| References: | 4 |
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| See Also: | See also related summary article "Development of improved turfgrass with herbicide, resistance and enhanced disease resistance through transformation" 1993 Turfgrass Research Summary [USGA], 1993, pp. 38-39, R=65327. R=65327 |
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| Note: | "November 1993"
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Last checked: 11/20/2013
Requires: PDF Reader |
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