| |
| Web URL(s): | http://archive.lib.msu.edu/tic/rpr/1990/24839,%20U%20Illinois,%20Smith.PDF
Last checked: 12/09/2013
Requires: PDF Reader
Notes: Link also includes "USGA Turfgrass Information File 1989-90" |
|---|
| Publication Type:
| Report |
|---|
| Material Type: | Manuscript |
|---|
| Monographic Author(s): | Smith, M. A. L. |
|---|
| Author Affiliation: | University of Illinois |
|---|
| Monograph Title: | [A Realistic Whole Plant Microculture Selection System for Turfgrasses: 1991 Annual Research Report], 1990. |
|---|
| Publishing Information: | [Champaign, Illinois]: University of Illinois |
|---|
| # of Pages: | 4 |
|---|
| Collation: | 4 pp. |
|---|
| Abstract/Contents: | "Superior salt and water deficit-tolerant turfgrass lines are needed for marginal planting sites, or irrigated sites where salt build-up is likely to occur. Effective selection of stress-tolerant genotypes is extremely complicated in the field due to environmental interactions that sometimes mask stress tolerance traits. Controlled environments can provide a more uniform test environment, to permit efficient germplasm prescreening and selection prior to scale-up for field evaluations. As part of this turfgrass research program, comparative, parallel studies to elucidate multiple symptoms of salt tolerance in warm and cool season turfgrasses have been completed in both solution culture and whole plant microculture. Plants are monitored from the small plug or seedling stage through an extended test period, to elucidate adaptations in both the root and shoot zone to increasing salt levels over time. Video image analysis uses a video camera and microcomputer to capture detailed, quantitative spatial [height, shoot area, root length and area] and spectral [visual density, color index] data on grass performance, and since the technique is completely non-destructive, plants can be effectively monitored as they adapt over time. The turfgrass responses to salt stress in both the in vitro and in vivo culture systems have shown excellent correlation, and have aided identification of key growth responses to stress, levels of salt that induce growth reductions, and timing before stress symptoms are evident. The objective visual data collected rapidly through image anaylsis has proven to be in strong agreement with conventional growth anaylsis of the treatments (the latter requries destructive sampling and dry weight measurement of plants). The experiments initiated in 1988 and 1989 were repeated in late 1989 and 1990 to provide additional replication of the experimental system data. In whole plant microculture, additional tests have separated the response characteristics of grasses when "shocked" by transplant to salt-supplemented sites, and when gradually adapted to those same salt concentrations. Evaluation of the recovery of the in vitro grass samples from salt stress is evaluated after they are transplanted back to non-salinized control media. The microculture stress-tolerance screens can be conducted on a smaller scale than field or greenhouse tests, do not require the high maintenance of growth chambers, and can be rapidly accomplished on a year-round basis. The tests will next be extended to novel selections which have been first screened for salt tolerance at the cell level, then regenerated into whole plants. This intermediate whole plant microculture step is an effective prescreen to determine whether cell-level traits are actually expressed in whole turfgrass plants." |
|---|
| Language: | English |
|---|
| References: | 0 |
|---|
| See Also: | See also related summary article "A realistic whole plant microculture selection system for turfgrasses" 1990 Annual Turfgrass Research Report [USGA/GCSAA], 1990, p. 11, R=24839. R=24839 |
|---|
| Note: | "Annual Research Report to the USGA - November 1990" |
|---|
| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
No defined citation format for TGIF #: 233877 |
| Fastlink to access this record outside TGIF: https://tic.lib.msu.edu/tgif/flink?recno=233877 |
| If there are problems with this record, send us feedback about record 233877. |
| Choices for finding the above item: |
|---|
| Web URL(s):
http://archive.lib.msu.edu/tic/rpr/1990/24839,%20U%20Illinois,%20Smith.PDF
Last checked: 12/09/2013
Requires: PDF Reader
Notes: Link also includes "USGA Turfgrass Information File 1989-90" |
| Request through your local library's inter-library loan service (bring or send a copy of this TGIF record) |
