| |
| Web URL(s): | http://archive.lib.msu.edu/tic/rpr/1990/24844,%20Ohio%20State,%20Shane.PDF
Last checked: 12/20/2013
Requires: PDF Reader |
|---|
| Publication Type:
| Report |
|---|
| Material Type: | Manuscript |
|---|
| Monographic Author(s): | Shane, William W.; Nameth, Stephen T. |
|---|
| Author Affiliation: | Shane: Project Leader, Principal Investigator and Turf Pathologist; and Nameth: Project Leader, Principle Investigator and Director, Plant and Pest Diagnostic Clinic |
|---|
| Monograph Title: | Monoclonal Antibodies for Rapid Diagnosis of Necrotic Ring Spot and Summer Patch: [1990 Annual Research Report], 1990. |
|---|
| Publishing Information: | Columbus, Ohio: Department of Plant Pathology, The Ohio State University |
|---|
| # of Pages: | 7 |
|---|
| Collation: | [7] pp. |
|---|
| Abstract/Contents: | "Slow-growing patch diseases are among the most difficult problems to diagnose on turfgrasses. Research at the Ohio State University, Department of Plant Pathology has focused on the development and use of immunological techniques for rapid diagnosis. We previously reported our success in developing a monoclonal antibody-producing clone that was selective for Leptosphaeria korrae, the causal agent of necrotic ring spot. The antibody, a small protein that can bind to the fungus, can now be grown in great quantity in a laboratory flask. The antibody allows us to test for the prescence of the pathogen in a plant sample. Our antibody was highly reactive against all fungal strains of Leptosphaeria korrae tested. The usefulness of the antibody for L. korrae has been tested thoroughly against diseased turfgrass submitted to the Ohio State University Plant and Pest Diagnostic Clinic and additional Kentucky bluegrass samples collected by our laboratory. L. korrae was successfully isolated from all Kentucky bluegrass samples exhibiting a significant reaction with the LK antibody. The LK antibody was used to study the distribution of L. korrae in the various regions of "frogeye" patches and on turfgrass plant parts. Sampling techniques for detection of L. korrae were optimized. The LK antibody successfully detected Leptosphaeria korrae in certain bermuda grass sites with spring dead spot symptoms. The antibody will be useful in determining the causal agent of spring dead spot. Currently, at least three fungi (L. korrae, Ophiosphaerella herpotricha, and Gaeumannomyces graminis) have been shown to be causes of this disease. Development of monoclonal antibody against the causal fungus of summer patch (Magnaporthe poae) is in progress. A third set of mice have been immunized using an improved protocol. Reactivity of mouse serum will be tested in November 1990, followed by production of monoclonal antibody clones. Screening of clones will begin in mid December, followed by field testing in the summer of 1991. The result will be a fast, reliable method to diagnose and monitor this disease." |
|---|
| Language: | English |
|---|
| References: | 0 |
|---|
| See Also: | See also related summary article "Monoclonal antibodies for rapid diagnosis of summer patch and necrotic ring spot diseases of turfgrasses" 1990 Annual Turfgrass Research Report [USGA/GCSAA], 1990, p. 18, R=24844. R=24844 |
|---|
| Note: | "October 29, 1990"
Tables |
|---|
| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
No defined citation format for TGIF #: 233908 |
| Fastlink to access this record outside TGIF: https://tic.lib.msu.edu/tgif/flink?recno=233908 |
| If there are problems with this record, send us feedback about record 233908. |
| Choices for finding the above item: |
|---|
| Web URL(s):
http://archive.lib.msu.edu/tic/rpr/1990/24844,%20Ohio%20State,%20Shane.PDF
Last checked: 12/20/2013
Requires: PDF Reader |
| Request through your local library's inter-library loan service (bring or send a copy of this TGIF record) |
