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| Web URL(s): | http://archive.lib.msu.edu/tic/rpr/1988/14551,%20Ohio%20State, Shane.PDF
Last checked: 04/29/2014
Requires: PDF Reader |
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| Publication Type:
| Report |
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| Material Type: | Manuscript |
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| Monographic Author(s): | Shane, William W.; Nameth, Stephen T. |
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| Author Affiliation: | Shane: Project Leader and Assistant Professor, Department of Plant Pathology, Ohio State University; and Nameth: Project Leader and Assistant Professor, Department of Plant Pathology, Ohio State University, and Director, Plant & Pest Diagnostic Clinic |
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| Monograph Title: | Monoclonal Antibodies for Rapid Diagnosis of Patch and Necrotic Ring Spot Diseases of Turfgrass: [1988 Annual Research Report], 1988. |
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| Publishing Information: | [Columbus, Ohio]: Department of Plant Pathology, Ohio State University |
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| # of Pages: | 16 |
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| Collation: | 12, [4] pp. |
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| Abstract/Contents: | "Summer patch and necrotic ring spot disease of Kentucky blue grass, annual blue grass, and other turf grasses are extremely difficult to diagnose with traditional techniques. Research at Ohio State University is focused on the use of immunological techniques for rapid diagnosis of these two diseases. Antibody-producing clones were developed for the causal agent of necrotic ring spot (Leptosphaeria korrae (LK)). Sixteen original clones were subcloned, purified, and screened against LK and non-LK fungi, soil, plant tissue. A single clone with the desired selectivity was increased for bulk antibody production. The final antibody reacted strongly against all verified strains of LK tested (including three from bermuda grass) and did not react significantly with 42 non-LK antigens (including Magnaporthe, Gaummanomyces, plant tissue, soil, and common plant-inhabiting fungi). Final refinements underway are: testing of LK anitbodies using field-infected plant material and optimizing assay procedures for routine clinic use. Similarly, monoclonal antibodies for summer patch (Magnaporthe poae) are under development. The first screen has been completed and indicated that the mouse antibodies react well with the original M. poae strain used for immunization. Subcloning, purification, and extensive screening are underway to acquire antibodies of the desired selectivity." |
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| Language: | English |
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| References: | 0 |
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| See Also: | See also related summary article "Monoclonal antibodies for rapid diagnosis of summer patch and necrotic ring spot diseases of turfgrasses" 1988 Turfgrass Research Summary [USGA/GCSAA], 1988, p. 24, R=14551. R=14551 |
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| Note: | "October 28, 1988"
Includes letter to William H. Bengeyfield from William W. Shane; p. [4]
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
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| Web URL(s):
http://archive.lib.msu.edu/tic/rpr/1988/14551,%20Ohio%20State, Shane.PDF
Last checked: 04/29/2014
Requires: PDF Reader |
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