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| DOI: | 10.2135/cropsci2016.04.0252 |
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| Web URL(s): | https://dl.sciencesocieties.org/publications/cs/articles/57/supplement1/S-179
Last checked: 08/18/2017
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https://dl.sciencesocieties.org/publications/cs/pdfs/57/supplement1/S-179
Last checked: 08/24/2017
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| Publication Type:
| Refereed |
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| Author(s): | Zhang, Chunzhen;
Fei, Shui-Zhang;
Liu, Peng;
Ji, Tieming;
Peng, Jiqing;
Frei, Ursula;
Hannapel, David J. |
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| Author Affiliation: | Zhang and Hannapel: Dep. of Horticulture, Iowa State Univ., Ames, IA; Liu and Ji: Dep. of Statistics, Iowa State Univ., Ames, IA; Frei: Plant Science Institute, Iowa State Univ., Ames, IA; Peng: GeneChip Facility, Dep. of Plant Pathology, Iowa State Univ., Ames, IA |
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| Title: | Transcriptome changes in response to cold acclimation in perennial ryegrass as revealed by a cross-species microarray analysis |
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| Section: | International Turfgrass Society Conference
Other records with the "International Turfgrass Society Conference" Section
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| Source: | Crop Science. Vol. 57, No. Supplement 1, July/August 2017, p. S-179-S-191. |
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| Publishing Information: | Madison, Wisconsin: Crop Science Society of America |
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| # of Pages: | 13 |
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| Related Web URL: | https://dl.sciencesocieties.org/publications/cs/abstracts/57/supplement1/S-179
Last checked: 08/18/2017
Notes: Abstract only
https://dl.sciencesocieties.org/publications/cs/supplements/0/cropsci2016-04-0252_supp-tables_supplement1.pdf
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https://dl.sciencesocieties.org/publications/cs/supplements/0/cropsci2016-04-0252_supp-figs_supplement2.pdf
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Notes: Supplemental figures |
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| Keywords: | TIC Keywords: Cold acclimation; Gene expression; Genetic analysis; Hordeum vulgare; Lolium perenne; Polymerase chain reaction; Ribonucleic acid
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| Abstract/Contents: | "Freezing tolerance in perennial ryegrass (Lolium perenne L.) can be dramatically increased by a period of cold acclimation. To understand the mechanisms of cold acclimation and freezing tolerance in L. perenne, a cross-species microarray study was conducted by using total RNA from cold-acclimated and nonacclimated L. perenne to hybridize with Affymetrix Barley1 GeneChips from barley (Hordeum vulgare L.). Our results indicate that selection of proper background correction and normalization method is critical for the success of a barleyperennial ryegrass cross-species microarray study. By using a specific three-step normalization method, a total of 2870 (12.6%) genes out of approximately 22,000 genes on the barley gene chip were identified as differentially regulated by either 1 or 7 d of cold acclimation. Quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) analysis confirmed that the expression patterns of 14 selected genes are consistent with the signal intensity patterns observed in the microarray study, supporting the validity of using a cross-species microarray in studying gene expression in species for which genomic resources remain scarce. Gene ontology analysis of differentially regulated genes indicates that different biological processes, cellular components, and molecular functions responded differently to cold acclimation in observable temporal patterns, suggesting that perennial ryegrass gradually acquires freezing tolerance by systematically regulating gene expression in an orderly manner." |
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| Language: | English |
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| References: | 41 |
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| Note: | Tables
Graphs |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Zhang, C., S.-Z. Fei, P. Liu, T. Ji, J. Peng, U. Frei, et al. 2017. Transcriptome changes in response to cold acclimation in perennial ryegrass as revealed by a cross-species microarray analysis. Crop Sci. 57(Supplement 1):p. S-179-S-191. |
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| DOI: 10.2135/cropsci2016.04.0252 |
| Web URL(s):
https://dl.sciencesocieties.org/publications/cs/articles/57/supplement1/S-179
Last checked: 08/18/2017
Access conditions: Item is within a limited-access website
https://dl.sciencesocieties.org/publications/cs/pdfs/57/supplement1/S-179
Last checked: 08/24/2017
Requires: PDF Reader
Access conditions: Item is within a limited-access website |
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MSU catalog number: b2211522a |
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