| |
| Web URL(s): | https://scisoc.confex.com/scisoc/2023am/meetingapp.cgi/Paper/151419
Last checked: 12/01/2023 |
|---|
| Publication Type:
| Report |
|---|
| Content Type: | Abstract or Summary only |
|---|
| Author(s): | Joseph, Mikerly;
Patel, Jinesh;
Rutland, Claudia Ann;
McElroy, J. Scott |
|---|
| Author Affiliation: | Joseph: Presenting Author and Agronomy, University of Florida, Gainesville, FL; Patel: Crop, Soil and Environmental Sciences, Auburn University, Auburn, AL; Rutland and McElroy: Auburn University, Auburn, AL |
|---|
| Title: | Differentiation between yellow foxtail (Setaria pumilla) and knotroot foxtail (Setaria parviflora) via morphological characteristics and genetic markers |
|---|
| Section: | Turfgrass breeding, genetics, molecular biology, microbiome oral I (includes student competition)
Other records with the "Turfgrass breeding, genetics, molecular biology, microbiome oral I (includes student competition)" Section
C05 turfgrass science
Other records with the "C05 turfgrass science" Section
|
|---|
| Meeting Info.: | St. Louis, Missouri: October 29-November 1, 2023 |
|---|
| Source: | ASA, CSSA, SSSA International Annual Meeting. 2023, p. 151419. |
|---|
| Publishing Information: | [Madison, Wisconsin]: [American Society of Agronomy, Crop Science Society of America, and Soil Science Society of America] |
|---|
| # of Pages: | 1 |
|---|
| Abstract/Contents: | "Weeds are primarily identified through morphological characteristics. However, weed identification using morphological aspects requires botanical expertise, and subtle differences between species can vary with subspecies or biotypic morphological differences. Setaria pumila (Poir.) Roem. & Schult. (1817) and Setaria parviflora (Poir.) Kerguelen, respectively known as yellow and knotroot foxtail, are problematic weed species that share morphological similarities and are often misidentified. Yellow and knotroot foxtail have phenotypic plasticity inter and intra-species, which can bias their identification. The criteria to differentiate those two species, such as the seedhead and rhizomes appears late in the growth stage, long after identification is required for herbicide management. Nucleic acids, DNA or RNA, are obvious options for identifying yellow and knotroot foxtail across biological systems beyond physical or digital identification. Research was conducted in the Herbicide Resistance Diagnostics Laboratory at Auburn University in Alabama to differentiate yellow and knotroot foxtail beyond morphological characteristics using DNA barcoding. 22 biotypes of yellow foxtail, 25 biotypes of knotroot foxtail, and 7 foxtails classifieds as unknown were identified and collected in Alabama using seedhead size, ligule size, and the presence of rhizomes. DNA for each biotype was isolated from the leaves and amplified using polymerase chain reaction (PCR) with primers targeting trnH-psbA, rbcl, matK, and ITS regions. Sanger sequencing results of the three-barcoding region, except psbA, demonstrate the presence of different single nucleotide polymorphisms in the sequenced regions that can differentiate yellow and knotroot foxtail. Some biotypes classified as unknown from their morphological characteristics were later correctly identified as yellow foxtail or knotroot foxtail by DNA barcoding. This study demonstrated that applying these DNA barcodes is relevant in accurately identifying foxtail species at an early stage and thus helps effectively manage them using proper herbicide selection." |
|---|
| Language: | English |
|---|
| References: | 0 |
|---|
| Note: | "221-10"
This item is an abstract only! |
|---|
| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Joseph, M., J. Patel, C. A. Rutland, and J. S. McElroy. 2023. Differentiation between yellow foxtail (Setaria pumilla) and knotroot foxtail (Setaria parviflora) via morphological characteristics and genetic markers. Agron. Abr. p. 151419. |
| Fastlink to access this record outside TGIF: https://tic.lib.msu.edu/tgif/flink?recno=333413 |
| If there are problems with this record, send us feedback about record 333413. |
| Choices for finding the above item: |
|---|
| Web URL(s):
https://scisoc.confex.com/scisoc/2023am/meetingapp.cgi/Paper/151419
Last checked: 12/01/2023 |
| Request through your local library's inter-library loan service (bring or send a copy of this TGIF record) |
