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| Web URL(s): | https://scisoc.confex.com/scisoc/2023am/meetingapp.cgi/Paper/150792
Last checked: 12/01/2023 |
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| Publication Type:
| Report |
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| Content Type: | Abstract or Summary only |
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| Author(s): | Chalise, Devendra Prasad;
Merewitz, Emily B. |
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| Author Affiliation: | Chalise: Presenting Author and Crop and Soil Sciences, Michigan State University, East Lansing, MI; Merewitz: Michigan State University, Michigan State University, East Lansing, MI |
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| Title: | Analysis of fluorescent and colorimetric dyes for perennial ryegrass organ viability: Visualization and quantification |
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| Section: | Turfgrass physiology, molecular biology, breeding, genetic and microbiome poster (includes student competition)
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| Meeting Info.: | St. Louis, Missouri: October 29-November 1, 2023 |
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| Source: | ASA, CSSA, SSSA International Annual Meeting. 2023, p. 150792. |
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| Publishing Information: | [Madison, Wisconsin]: [American Society of Agronomy, Crop Science Society of America, and Soil Science Society of America] |
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| # of Pages: | 1 |
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| Abstract/Contents: | "Perennial ryegrass (Lolium perenne) is a vital turf and forage grass species in temperate regions; however, its growing region is limited by winterkill susceptibility. Crown tissues are the primary overwintering structure in many perennial grass species, thus research to specifically evaluate crown viability methodology is needed. The triphenyl tetrazolium chloride (TTC) method is commonly used for roots and seeds but little research has investigated the method for grass crown viability. This study aimed to assess the viability of perennial ryegrass roots and crowns using fluorescent dyes (SYTOX blue, propidium iodide (PI), and fluorescein diacetate (FDA)) and spectrophotometric dyes (TTC, evans blue, trypan blue, alamar blue, and neutral red). Live and dead tissues of roots and crowns were used, and dead tissues were obtained by heating them at 100°C for 30 minutes in an oven. PI and SYTOX blue revealed distinct fluorescent color disparities between live and dead tissues, with dead tissues exhibiting higher fluorescence (67.13% and 74.34% for PI, 28.84% and 58.01% for SYTOX blue in root and crown, respectively). TTC showed a strong correlation between live weight (%) and optical density per gram (R2 = 0.9319 for roots and R2 = 0.9398 for crowns). These findings highlight PI and SYTOX blue's potential in distinguishing between healthy and damaged perennial ryegrass at the microscopic level to aid in mechanistic research. The strong correlation of TTC with viability indicates its utility for crown tissues using spectrophotometer methods. Future research should explore these techniques to enhance viability assessment precision, particularly under diverse environmental stress conditions." |
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| Language: | English |
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| References: | 0 |
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| Note: | This item is an abstract only! |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Chalise, D. P., and E. B. Merewitz. 2023. Analysis of fluorescent and colorimetric dyes for perennial ryegrass organ viability: Visualization and quantification. Agron. Abr. p. 150792. |
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| Web URL(s):
https://scisoc.confex.com/scisoc/2023am/meetingapp.cgi/Paper/150792
Last checked: 12/01/2023 |
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