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| Web URL(s): | https://scisoc.confex.com/scisoc/2023am/meetingapp.cgi/Paper/151686
Last checked: 12/01/2023 |
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| Publication Type:
| Report |
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| Content Type: | Abstract or Summary only |
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| Author(s): | Dhillon, Hamalpreet Kaur;
Vines, Phillip L.;
Schwartz, Brian M. |
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| Author Affiliation: | Dhillon: Presenting Author and Institute of Plant Breeding, Genetics & Genomics, University of Georgia, Tifton, GA; Vines and Schwartz: Department of Crop and Soil Sciences, University of Georgia, Tifton, GA |
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| Title: | Breeding better zoysiagrass: Harnessing somaclonal variation to enhance seed and pollen sterility through tissue culture |
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| Section: | Turfgrass physiology, molecular biology, breeding, genetic and microbiome poster (includes student competition)
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| Meeting Info.: | St. Louis, Missouri: October 29-November 1, 2023 |
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| Source: | ASA, CSSA, SSSA International Annual Meeting. 2023, p. 151686. |
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| Publishing Information: | [Madison, Wisconsin]: [American Society of Agronomy, Crop Science Society of America, and Soil Science Society of America] |
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| # of Pages: | 1 |
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| Abstract/Contents: | "Zoysiagrasses (Zoysia spp. Willd.) are allotetraploid (2n=4x=40) perennial warm season grasses that require little maintenance and tolerate drought, low soil fertility, and saline environments. However, the presence of fertile inflorescence in zoysiagrass can lead to unwanted seedling establishment and cross pollination with other grasses. To address this issue, we explored the use of tissue culture to induce somaclonal variation in an effort to induce sterility in zoysiagrass. We tested different explants, including seeds, nodes and immature inflorescence to establish callus cultures. Different explants were cultured on basic Murashige and Skoog medium, 3% sucrose, 0.25% gelrite, 4 mg/L Thiamine-HCl, 100 mg/L α-Ketoglutaric acid, 2 mL/L Plant Preservative Mixture supplemented with different concentrations of growth hormones- for seeds and immature inflorescence (1 mg/L to 5 mg/L 2,4-D, 0.01 mg/L to 0.1 mg/L 6-Benzyladenine), for nodes (2 mg/L to 5 mg/L 2,4-D, 0.1 mg/L 6-Benzyladenine, with or without 0.0082 mg/L Abscisic acid), pH-5.8. All the explants were cultured in the dark at 26±°C for 1 month (nodes), 2 months (immature inflorescence), 3 months (seeds) for callus induction. The frequency of callus formation varied among different explants with highest in case of seeds (44%) followed by nodes (7%) and then by immature inflorescence (2%). The calli were further classified into three types: type I as embryogenic with bright yellow color and firm texture; type II callus as friable without secretion; type III callus as white, highly friable, and non-embryogenic. Type I and II embryogenic calluses were transferred to regeneration media (Murashige and Skoog medium, 3% maltose, 0.25% gelrite, 0.05mg/L naphthalene acetic acid, 0.1mg/L gibberellic acid, 1mg/L thidiazuron) for shoot and root induction and after formation of complete plant they will be transferred to soil and through clonal propagation their phenotype will be observed for pollen and seed sterility in multiple environments over time." |
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| Language: | English |
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| References: | 0 |
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| See Also: | Updated version appears in Golf Course Management, January 2024, p.103, R=334118 R=334118 |
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| Note: | This item is an abstract only! |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Dhillon, K. K., P. L. Vines, and B. M. Schwartz. 2023. Breeding better zoysiagrass: Harnessing somaclonal variation to enhance seed and pollen sterility through tissue culture. Agron. Abr. p. 151686. |
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| Web URL(s):
https://scisoc.confex.com/scisoc/2023am/meetingapp.cgi/Paper/151686
Last checked: 12/01/2023 |
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