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| Web URL(s): | https://scisoc.confex.com/scisoc/2023am/meetingapp.cgi/Paper/153775
Last checked: 12/04/2023
https://scisoc.confex.com/scisoc/2023am/meetingapp.cgi/Paper/153783
Last checked: 12/06/2023
Notes: ASA, CSSA, SSSA International Annual Meeting reprint |
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| Publication Type:
| Report |
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| Content Type: | Abstract or Summary only |
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| Author(s): | Suazo Mejia, Jose;
Buhlman, Jamie L.;
Kenworthy, Kevin E. |
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| Author Affiliation: | Suazo Mejia: Presenting Author and University of Florida, Gainesville, FL; Buhlman: Agronomy, University of Florida, Gainesville, FL; Kenworthy: Department of Agronomy, University of Florida, Gainesville, FL |
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| Title: | Increasing ploidy in frogfruit (Phyla nodiflora L. Greene) seedlings using colchicine |
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| Section: | Turfgrass physiology, molecular biology, breeding, genetic and microbiome poster (includes student competition)
Other records with the "Turfgrass physiology, molecular biology, breeding, genetic and microbiome poster (includes student competition)" Section
C05 turfgrass science
Other records with the "C05 turfgrass science" Section
210
Other records with the "210" Section
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| Meeting Info.: | St. Louis, Missouri: October 29-November 1, 2023 |
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| Source: | ASA, CSSA, SSSA International Annual Meeting. 2023, p. 153775. |
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| Publishing Information: | [Madison, Wisconsin]: [American Society of Agronomy, Crop Science Society of America, and Soil Science Society of America] |
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| # of Pages: | 1 |
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| Abstract/Contents: | "There is increasing interest in Florida for ground cover alternatives to traditional turfgrass lawns. Frogfruit (Phyla nodiflora L. Greene) has garnered significant attention as a potential ground cover due to prostrate growth habit, resilience, and its status as a Florida native. Frogfruit is a perennial herbaceous dicotyledonous plant with distinctive white and pink flowers that are visited by a wide range of pollinators. Additionally, it produces an attractive ornamental groundcover that is also tolerant of mowing. Frogfruit has also been noted to withstand many environmental challenges, such as drought, high salinity, and high traffic. Due to its many benefits and overall hardiness, it merits consideration for use in landscapes and breeding for improvement. Chromosome doubling has been used to increase plant morphological characteristics, including flower size, to increase attractiveness to consumers. The objective of this experiment was to induce polyploidy in frogfruit, which is naturally diploid. Germinating frogfruit seeds were treated for two hours with either a 1% or 5% colchicine solution, grown in petri dishes, and subsequently transplanted into pots. To confirm a successful increase in ploidy, several plants with larger morphological features were selectively compared to their wild-type parents to evaluate the number and size of stomata, and flow cytometry was performed to estimate ploidy of the mutant progeny. Results indicated that an increase in ploidy was successfully achieved as several mutant phenotypes were produced in colchicine treated seedlings. Mutant phenotypes included larger, wider, and thicker leaves, as well as thicker stems and larger flowers. The stomatal size of the mutants varied with some having stomates twice the size of wild-type parents. Mutant plants also had higher picogram/nuclei values providing strong evidence that these methods were successful for induction of polyploidy in frogfruit." |
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| Language: | English |
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| References: | 0 |
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| Note: | Reprint appears in ASA, CSSA, SSSA International Annual Meeting, 2023, p. 153783, with variant section 302-7- Turfgrass breeding, genetic, molecular biology, microbiome oral II
This item is an abstract only! |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Suazo Mejia, J., J. L. Buhlman, and K. E. Kenworthy. 2023. Increasing ploidy in frogfruit (Phyla nodiflora L. Greene) seedlings using colchicine. Agron. Abr. p. 153775. |
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| Web URL(s):
https://scisoc.confex.com/scisoc/2023am/meetingapp.cgi/Paper/153775
Last checked: 12/04/2023
https://scisoc.confex.com/scisoc/2023am/meetingapp.cgi/Paper/153783
Last checked: 12/06/2023
Notes: ASA, CSSA, SSSA International Annual Meeting reprint |
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