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| Web URL(s): | https://scisoc.confex.com/scisoc/2023am/meetingapp.cgi/Paper/150486
Last checked: 12/07/2023 |
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| Publication Type:
| Report |
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| Content Type: | Abstract or Summary only |
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| Author(s): | Tucker, Matthew;
McCall, David S.;
Eisenback, Jon D. |
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| Author Affiliation: | Tucker: Presenting Author and Virginia Tech, Blacksburg, VA; McCall and Eisenback: School of Plant and Environmental Sciences, Virginia Tech, Blacksburg, VA |
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| Title: | Interlab variability of nematode assays from turfgrass systems |
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| Section: | 377
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| Meeting Info.: | St. Louis, Missouri: October 29-November 1, 2023 |
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| Source: | ASA, CSSA, SSSA International Annual Meeting. 2023, p. 150486. |
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| Publishing Information: | [Madison, Wisconsin]: [American Society of Agronomy, Crop Science Society of America, and Soil Science Society of America] |
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| # of Pages: | 1 |
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| Abstract/Contents: | "Nematode assay extraction methods vary by lab for quantifying plant-parasitic nematodes (PPN) from turfgrass samples. The objective of this research was to compare the relative extraction efficiencies of homogenous turfgrass samples by various state nematode labs. Random samples were collected from three locations across the Mid-Atlantic United States, homogenized, and divided into thirty 500 cc subsamples. Three subsamples from each location were sent to ten state nematode assay labs (n=90). Semi-automatic elutriation (n=27) was used to extract PPN by three labs; Louisiana, North Carolina, and Virginia. Nematodes were extracted by hand sieving (n=63) in the remaining seven labs; Alabama, Florida, Georgia, Massachusetts, Michigan, Mississippi, and South Carolina. Six PPN genera identified across all labs included Hoplolaimus, Mesocriconema, Tylenchorynchus, Meloidogyne, Helicotylenchus, and Paratrichodorus. Five or six genera of PPN were different by state where Helicotylenchus was similar across states due to low or no populations present. Estimated populations of PPN varied among states with the same extraction methods. Extractions using semi-automatic elutriation were similar among the three tested labs for all genera except Hoplolaimus, where estimated populations were higher from the Virginia lab than from Louisiana or North Carolina labs. Estimated populations were significantly different for four of six genera when evaluated using the hand-sieving method. Analysis by method suggests that a component of automation may reduce variability among PPN counts. Ultimately, this research demonstrates the need to use the same lab over time for the most consistent PPN information." |
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| Language: | English |
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| References: | 0 |
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| Note: | This item is an abstract only! |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Tucker, M., D. S. McCall, and J. D. Eisenback. 2023. Interlab variability of nematode assays from turfgrass systems. Agron. Abr. p. 150486. |
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| Web URL(s):
https://scisoc.confex.com/scisoc/2023am/meetingapp.cgi/Paper/150486
Last checked: 12/07/2023 |
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