| |
Web URL(s): | http://archive.lib.msu.edu/tic/ressuml/210.pdf Last checked: 01/20/2017 Requires: PDF Reader |
Publication Type:
| Report |
Author(s): | Hughes, Harrison G. |
Author Affiliation: | Colorado State University |
Title: | Long-term preservation of clonally propagated turfgrass species |
Section: | Turfgrass germplasm enhancement Other records with the "Turfgrass germplasm enhancement" Section
|
Source: | 2000 Turfgrass and Environmental Research Summary [USGA]. 2000, p. 38. |
Publishing Information: | Far Hills, NJ: United States Golf Association |
# of Pages: | 1 |
Full Report URL: | http://turf.lib.msu.edu/rprl/616.pdf Last checked: 9/2001 Requires: Adobe Acrobat Notes: This is the entire full report! |
Keywords: | TIC Keywords: Germplasm; Polycross progeny; Micropropagation; Cynodon dactylon; Zoysia; Distichlis spicata; Bouteloua dactyloides; Root tips; Germination media; Tissue culture; Cryogenic endodormancy; Cryogenic paradormancy; Freezing; Timing; Golf courses
|
Abstract/Contents: | Presents a study with the following objectives: "1. Develop suitable micropropagation procedures for selected genotypes of bermudagrass, zoysiagrass, saltgrass and buffalograss. 2. Develop suitable shoot tip culture media (SCTM) for the four species. 3. Examine cryopreservation of the four species using vitrificiation methodologies." States that "three cryopreservation methods are being tested: two-step cooling, vitrification, and encapsulation/dehydration." Reports that although clean cultures have been established with both saltgrass and bermudagrass, the proliferation rates are minimal." Also, "buffalograss has been cryopreserved with the vitrification system." This three-year study began in 1998. |
Language: | English |
References: | 0 |
See Also: | See also related manuscript, Long-Term Preservation of Clonally Propagated Turfgrass Species, 2000, R=215147. R=215147 |
See Also: | Other Reports from this USGA research project: 1998-20-128 |
Note: | Pictures, color |
USGA Summary Points: | Three cryopreservation methods are being tested: two-step cooling, vitrification, and encapsulation/dehydration. Cooling rate was important - more rapidly cooled shoot tips showed higher levels of survival. Attention to quality of shoot tips is important - need axillary meristems. For buffalograss, vitrification had worked the best. Prograss is being made to cryopreserve bermudagrass and saltgrass. Micropropagation systems for buffalograss and zoysiagrass are needed that will allow sufficient proliferation rates for cryopreservation studies. |
| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete): Hughes, H. G. 2000. Long-term preservation of clonally propagated turfgrass species. Turfgrass Environ Res. Summ. p. 38. |
| Fastlink to access this record outside TGIF: https://tic.lib.msu.edu/tgif/flink?recno=72140 |
| If there are problems with this record, send us feedback about record 72140. |
| Choices for finding the above item: |
| Web URL(s): http://turf.lib.msu.edu/rprl/616.pdf Last checked: 9/2001 Requires: Adobe Acrobat Notes: This is the entire full report! |
| MSU catalog number: SB 433 .A1 A6 |
| Request through your local library's inter-library loan service (bring or send a copy of this TGIF record) |