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Web URL(s): | http://archive.lib.msu.edu/tic/ressuml/223.pdf Last checked: 01/20/2017 Requires: PDF Reader |
Publication Type:
| Report |
Author(s): | Meyer, William A. |
Author Affiliation: | Rutger University |
Title: | Identification of creeping bentgrass (Agrostis palustris Huds.) cultivars using simple sequence repeats (SSRs) |
Section: | Turfgrass germplasm enhancement Other records with the "Turfgrass germplasm enhancement" Section
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Source: | 2000 Turfgrass and Environmental Research Summary [USGA]. 2000, p. 51. |
Publishing Information: | Far Hills, NJ: United States Golf Association |
# of Pages: | 1 |
Full Report URL: | http://turf.lib.msu.edu/rprl/630.pdf Last checked: 9/2001 Requires: Adobe Acrobat Notes: This is the entire full report! |
Keywords: | TIC Keywords: Methodology; Techniques; Agrostis stolonifera; Simple sequence repeats; Cultivar identification; Genetic diversity; Cross pollination; Loci; Identification; Golf courses
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Abstract/Contents: | Presents a study with the following objectives: "1. Construct a DNA library enriched for selected simple sequence repeats (SSRs). 2. Isolate and characterize approximately 30 loci from the SSR enriched creeping bentgrass DNA library. 3. Use SSRs to explore diversity within closely and distantly related creeping bentgrass cultivars. 4. Explore genetic relationships among creeping bentgrass cultivars. 5. Determine the optimum sample size needed to represent cross-pollinated cultivars. 6. Assess the utility of isolated SSR loci for use within other Agrostis species." States that "we have already studied the utility of SSRs for cultivar identification in perennial ryegrass and found them useful for establishing genetic relationships and for distinguishing closely related perennial ryegrass clones. We hav also determined that the optimum sample size needed to represent a perennial ryegrass cultivar is at least 20 individuals and the optimum number of SSR loci necessary to represent a cultivar is 15. This work should be applicable to our studies in creeping bentgrass." This two-year study began in 2000. |
Language: | English |
References: | 0 |
See Also: | See also related manuscript, Identification of Creeping Bentgrass (Agrostis palustris Huds.) Cultivars Using Simple Sequence Repeats (SSRs), 2000, R=72153. R=72153 |
See Also: | Other Reports from this USGA research project: 2000-07-174 |
Note: | Pictures, color |
USGA Summary Points: | Only two bentgrass SSRs have been produced. More than 30 are needed to detect differences between closely related cultivars. The optimum sample size needed to represent a perennial ryegrass cultivar is at least 20 individuals and the optimum number of SSR loci necessary to adequately distinguish the cultivar is 15. |
| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete): Meyer, W. A. 2000. Identification of creeping bentgrass (Agrostis palustris Huds.) cultivars using simple sequence repeats (SSRs). Turfgrass Environ Res. Summ. p. 51. |
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| Web URL(s): http://turf.lib.msu.edu/rprl/630.pdf Last checked: 9/2001 Requires: Adobe Acrobat Notes: This is the entire full report! |
| MSU catalog number: SB 433 .A1 A6 |
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