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| Web URL(s): | http://archive.lib.msu.edu/tic/rpr/2000/72138,%20Michigan State, Sticklen.PDF
Last checked: 02/08/2013
Requires: PDF Reader |
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| Publication Type:
| Report |
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| Material Type: | Manuscript |
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| Monographic Author(s): | Sticklen, Mariam; Vargas, Joseph |
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| Author Affiliation: | Sticklen: Professor, Crop & Soil Sciences Dept.; and Vargas: Professor, Bot. & Plant Pathol. Dept., Michigan State University, East Lansing, MI |
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| Monograph Title: | A Multigene-Transfer Strategy to Control Pathogens and Enhance Environmental Stress Tolerance in Creeping Bentgrass: 2000 Annual Report, 2000. |
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| Publishing Information: | East Lansing, Michigan: Michigan State University |
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| # of Pages: | 7 |
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| Collation: | [7] pp. |
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| Abstract/Contents: | "Previously, Sticklen's research team developed creeping bentgrass clones that contain the glufosinate ammonia (Liberty or FinaleTM) resistant herbicide, a chitinae gene, a proteinase inhibitor gene, and a drought and salt tolerance mannitol dehydrogenase (mtlD) gene. Studies have shown that the chitinase genes can make transgenic plants resistant to pathogenic fungi such as R. solani, etc.. Our research team confirmed that glofosinate ammonia has fungicidal as well as herbicidal properties. Therefore, we have been able to simultaneously control weeds as well as turfgrass pathogens (mainly Sclerotinia ulnocarpal and Rhizoctonia solani) by spaying this herbicide on transgenic creeping bentgrass expressing the bar gene under greenhouse conditions. Research by Dr. Vargas laboratory has shown two out of 44 independently transformed lines of our transgenic creeping bentgrass, transcribing the elm chitinase gene, has improved resistance of plants to R. solani under controlled environmental conditions. Experiments by Dr. Baird's laboratory has shown no translation of our transcribed mtlD gene in certain lines of transgenic turfgrass. Of course, none of these transgenic lines tested for western blotting also showed accumulation of mannitol, as a sign for drought and/or salt tolerance. With no surprise, none of these transgenic lines tested showed any drought and/or salt tolerance either. Sine we made synthetic prptides and produced extra antibodies to the MTLD protein, we plan to test the rest of mtlD-transcribed transgenic lines for expression of MTLD protein by western blotting. If positive, the one again we will perform mannitol test and will also test plants for drought and or salt tolerance. Should MSU have a new turfgrass physiologist in place soon. this work will be performed with collaboration of the new physiologist." |
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| Language: | English |
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| References: | 22 |
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| See Also: | See also related summary article, "A multigene-transfer strategy to improve disease and environmental stress resistance in creeping bentgrass", 2000 Turfgrass and Environmental Research Summary [USGA], 2000, p. 36, R=72138. R=72138 |
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| Note: | "2000 Annual Report"
"Dr. James Baird was one of the Co-PIs of this proposal. However, he has not been involved with this final report because he is no more at MSU." |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
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Last checked: 02/08/2013
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