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| Web URL(s): | http://archive.lib.msu.edu/tic/rpr/2000/72142,%20%20NC%20State,%20Qu.PDF
Last checked: 02/08/2013
Requires: PDF Reader |
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| Publication Type:
| Report |
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| Material Type: | Manuscript |
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| Monographic Author(s): | Li, Liangcai; Qu, Rongda; Davis, Eric; Schubert, Karel |
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| Author Affiliation: | Qu: Principal Investigator; Li: Visiting Scientist; Davis: Assistant Professor, Plant Pathology, NCSU; and Schubert: Professor, Botany, University of Oklahoma |
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| Monograph Title: | Hybrid Bermudagrass Improvement by Genetic Transformation: 2000 Annual Report to United States Golf Association, 2000. |
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| Publishing Information: | [Raleigh, North Carolina]: Department of Crop Science, North Carolina State University |
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| # of Pages: | 11 |
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| Collation: | 6, [5] pp. |
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| Abstract/Contents: | "The ultimate goal of this research project is to improve turf-type bermudagrass cultivars for the golf courses through biotechnology. The specific goals of the project regenerating plantlets of bermudagrass, to develop procedures to transform bermudagrass, and to obtain transgenic bermudagrass plants that express potential nematode resistant genes. The turf-type bermudagrass is overall recalcitrant for plant tissue culture. We mainly use the young inflorescences of hybrid cultivar 'Tifgreen' and a common bermudagrass cultivar 'Savannah' as tissue culture materials because their potential to regenerate. So far, we have spent substantial efforts to improve the tissue culture responses of bermudagrass. We have showed previously that by supplementing 6-benzylaminopurine (BAP) and abscisic acid (ABA) in the callus induction medium, we substantially improved somatic embryogenesis from bermudagrass calluses. Addition of gibberellic acid further improved the germination/regeneration of the embryos. This year, we repeated the experiments and confirmed our observation. We also observed secondary somatic embryogenesis in bermudagrass culture which contributed to the regeneration. Moreover, we found a better combination of phytohormones for common bermudagrass tissue culture medium. We have spent most of our efforts in the transformation experiments with emphasis on Agrobacterium-mediated transformation. A gene expression vector pRQ219 was constructed and 8 Agrobacterium strains were made. We found that some strains are more infectious in bermudagrass than others, and some bermudagrass cultivars are more susceptible than other cultivars. We also identified the optimal time to perform the infection. We performed 28 batches of agro-transformation experiments using young inflorescences and their derived calluses. In addition, we established 5 suspension cell lines which have been used for transformation experiments. Using an intron-GUS reporter gene, we observed cell clusters showing GUS activity, indicating stable transformation of bermudagrass cells." |
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| Language: | English |
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| References: | 0 |
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| See Also: | See also related summary article, "Hybrid bermudagrass improvement by genetic transformation", 2000 Turfgrass and Environmental Research Summary [USGA], 2000, p. 40, R=72142. R=72142 |
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| Note: | Pictures, b/w
Figures
Tables
"November 1, 2000"
"2000 Annual Report to United States Golf Association"
Also appears as pp. 427-437 in the USGA Turfgrass Research Committee Reporting Binders for 2000. |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
No defined citation format for TGIF #: 215154 |
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Last checked: 02/08/2013
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