| |
| Web URL(s): | http://archive.lib.msu.edu/tic/rpr/1994/35313,%20U%20Florida, Giblin-Davis.PDF
Last checked: 11/14/2013
Requires: PDF Reader |
|---|
| Publication Type:
| Report |
|---|
| Material Type: | Manuscript |
|---|
| Monographic Author(s): | Giblin-Davis, Robin M.; Dickson, Don W.; Cisar, John L. |
|---|
| Author Affiliation: | Giblin-Davis and Cisar: Ft. Lauderdale Research and Education Center, University of Florida, Ft. Lauderdale; and Giblin-Davis and Dickson: Department of Entomology and Nematology, University of Florida, Gainesville, FL |
|---|
| Monograph Title: | Pasteuria sp. for Biological Control of the Sting Nematode, Belonolaimus longicaudatus, in Turfgrass: [1994 Annual Research Report], 1994. |
|---|
| Publishing Information: | Ft. Lauderdale, Florida; Gainesville, Florida: University of Florida |
|---|
| # of Pages: | 15 |
|---|
| Collation: | 7, [8] pp. |
|---|
| Abstract/Contents: | "We are describing a new species of bacterium in the genus, Pasteuria that we discovered parasitizing the sting nematode, Belonolaimus longicaudatus in Florida. We are hopeful that this obligate bacterial parasite of nematodes (Pasteuria n. sp. [S-1]) will have some potential for inoculative biological control in golf course greens against the sting nematode; a destructive ectoparasite that can reduce the root dry weight of turfgrasses and other crops in sandy soils by as much as 30-50%. In 1994, we completed ultrastructural studies with transmission electron microscopy (TEM) that show that Pasteuria n. sp. (S-1) is a new species and have helped to elucidate the life cycle. The sporangium and endospore diameters of Pasteuria n. sp. (S-1) are on the average at least 1.0 and 0.5 μm wider than these respective measurements for the other described species of Pasteuria. The epicortical wall of Pasteuria n. sp. (S-1) surrounds the endospore in sublateral band and the basal cortical wall thins to expose the inner endospore, similar to P. thornei but different from the other two described spices. The outer cortical wall thickness at its thickest point is 1/3 the endospore diameter for Pasteuria n. sp. (S-1) compared with ¼-1/15 for the other described species of Pasteuria. A brief description of the life cycle follows (Fig. 1); after attachment of a mature endospore to the cuticle of the host, penetration ensues via a germ tube through the cuticle into the pseudocoelom of the nematode. A mycelial microcolony is formed which eventually breaks up and is distributed throughout the pseudocoelom (fragmentation). Mycelial filaments are divided by septa and possess double-layered cell walls. Endospores are produced endogenously and the formation sequence (sporogenesis) for Pasteuria n. sp. (S-1) is similar to the three other described species of Pasteuria. A septum is formed within the sporangium, the sporangium cytoplast condenses to form a forespore, the endospore walls form, the endospore matures, and areas adjacent to the endospore give rise to perisporal "attachment" fibers. Laboratory host attachment studies and field observations done in 1994 on Pasteuria n. sp, (S-1) demonstrate that it is highly host specific and attacks only nematodes in the genus Belonolaimus or within the species, B. longicaudatus. We have initiated population dynamic studies on Pasteuria n. sp. (S-1) in laboratory pot cultures of the sting nematode on the model turfgrass host (FX-313 St. Augustinegrass) under controlled conditions. After 84 days, sting nematode cultures which were inoculated with 10 or 25 sting nematodes with Pasteuria n. sp. (S-1) have not shown suppression or a disease epizootic. The experiment will run for at least 210 days and it may take at least this long for establishment of the bacteria under these conditions. In 1995, we propose to do studies on the effects of temperature on development of Pasteuria n. sp. (S-1), monthly survey work in golf course areas where this bacterium occurs naturally to assess its suppressive effects on sting nematodes and to begin sampling golf course areas where sting nematode is no longer a problem to try and isolate different species of isolates of antagonists to nematodes of turfgrass." |
|---|
| Language: | English |
|---|
| References: | 0 |
|---|
| See Also: | See also related summary article "Pasteuria sp. for biological control of the sting nematode, (Belonolaimus longicaudatus), in turfgrass" 1994 Turfgrass Research Summary [USGA], 1994, p. 54-55, R=35313. R=35313 |
|---|
| Note: | "1994 USGA Turfgrass Research Report"
Pictures, b/w
Figures
Tables
Graphs |
|---|
| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
No defined citation format for TGIF #: 232608 |
| Fastlink to access this record outside TGIF: https://tic.lib.msu.edu/tgif/flink?recno=232608 |
| If there are problems with this record, send us feedback about record 232608. |
| Choices for finding the above item: |
|---|
| Web URL(s):
http://archive.lib.msu.edu/tic/rpr/1994/35313,%20U%20Florida, Giblin-Davis.PDF
Last checked: 11/14/2013
Requires: PDF Reader |
| Request through your local library's inter-library loan service (bring or send a copy of this TGIF record) |
